Establishment of experimental periapical inflammatory lesions in mice

Naoto Osuga, Saeka Matsuda, Masahito Shoumura, Keita Moriyama, Yukiko Yokoi, Keisuke Nakano, Toshiyuki Kawakami

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Although studies on the formation of apical periodontitis have somehow been carried out but detailed cellular dynamics remain unclear. We recently established an experiment that could easily be performed using ddY mice. First, under general anesthesia using isoflurane inhalation, the coronal portion of the maxillary first molar was penetrated using a round bur and drill with water irrigation causing pulp exposure until the root apex. Micro computed tomography (R_mCT) was taken over time during observation. Four weeks later, R_mCT confirmed the presence of a radiolucent image at the apex of the tooth, which was then removed for histological examination. The results showed that granulation tissue with fibrosis had gradually formed at the periphery of the abscess. The present method confirmed the effectiveness of the experimental mode to exmine the formation of chronic inflammatory lesions at the root apex.

Original languageEnglish
Pages (from-to)517-520
Number of pages4
JournalJournal of Hard Tissue Biology
Issue number4
Publication statusPublished - 2013
Externally publishedYes


  • Experimental model
  • Histopathology
  • Inflammatory apical periodontitis
  • Micro-CT
  • Mouse

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • General Dentistry
  • Cell Biology


Dive into the research topics of 'Establishment of experimental periapical inflammatory lesions in mice'. Together they form a unique fingerprint.

Cite this