Establishment of ndart1-tagged lines of koshihikari, an elite variety of rice in japan

Hideki Nishimura; Eiko Himi; Kazuhide Rikiishi; Kazuo Tsugane; Masahiko Maekawa

doi:10.1270/jsbbs.19049

Establishment of ndart1-tagged lines of koshihikari, an elite variety of rice in japan

Hideki Nishimura, Eiko Himi, Kazuhide Rikiishi, Kazuo Tsugane, Masahiko Maekawa

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.

Original language	English
Pages (from-to)	696-701
Number of pages	6
Journal	Breeding Science
Volume	69
Issue number	4
DOIs	https://doi.org/10.1270/jsbbs.19049
Publication status	Published - 2019

Keywords

DNA transposon
Insertional mutagenesis
Koshihikari
NDart1-tagged lines
Rice

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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10.1270/jsbbs.19049

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title = "Establishment of ndart1-tagged lines of koshihikari, an elite variety of rice in japan",

abstract = "To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.",

keywords = "DNA transposon, Insertional mutagenesis, Koshihikari, NDart1-tagged lines, Rice",

author = "Hideki Nishimura and Eiko Himi and Kazuhide Rikiishi and Kazuo Tsugane and Masahiko Maekawa",

note = "Funding Information: We thank Ms. Seiko Nakano and Ms. Yoko Kobayashi (IPSR) for their technical support. This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan [No. 16K07561] and the Joint Research Program implemented at the Institute of Plant Science and Resources, Okayama University, Japan (No. 2942 to K.T.), and the NIBB Cooperative Research Program (17-331 to M.M.). Publisher Copyright: {\textcopyright} 2019, Japanese Society of Breeding. All rights reserved.",

year = "2019",

doi = "10.1270/jsbbs.19049",

language = "English",

volume = "69",

pages = "696--701",

journal = "Breeding Science",

issn = "1344-7610",

publisher = "Japanese Society of Breeding",

number = "4",

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T1 - Establishment of ndart1-tagged lines of koshihikari, an elite variety of rice in japan

AU - Nishimura, Hideki

AU - Himi, Eiko

AU - Rikiishi, Kazuhide

AU - Tsugane, Kazuo

AU - Maekawa, Masahiko

N1 - Funding Information: We thank Ms. Seiko Nakano and Ms. Yoko Kobayashi (IPSR) for their technical support. This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan [No. 16K07561] and the Joint Research Program implemented at the Institute of Plant Science and Resources, Okayama University, Japan (No. 2942 to K.T.), and the NIBB Cooperative Research Program (17-331 to M.M.). Publisher Copyright: © 2019, Japanese Society of Breeding. All rights reserved.

PY - 2019

Y1 - 2019

N2 - To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.

AB - To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.

KW - DNA transposon

KW - Insertional mutagenesis

KW - Koshihikari

KW - NDart1-tagged lines

KW - Rice

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Establishment of ndart1-tagged lines of koshihikari, an elite variety of rice in japan

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Keywords

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