Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method

Shinichi Usui; Tetsuya Mizuno; Mitsuyo Okazaki; Masakazu Nakamura; Ikunosuke Sakurabayashi

doi:10.1016/j.clinbiochem.2008.10.007

Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method

Shinichi Usui, Tetsuya Mizuno, Mitsuyo Okazaki, Masakazu Nakamura, Ikunosuke Sakurabayashi

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Objectives: To evaluate the gel permeation high-performance liquid chromatography (GP-HPLC) method for determination of triglyceride (TG) levels in low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Design and methods: The GP-HPLC and the ultracentrifugation(UC)/precipitation methods were used and compared. Results: There was no significant difference in measured levels of LDL-triglyceride between the two methods, but the HDL-triglyceride levels measured by the GP-HPLC technique were significantly higher than the UC/precipitation one (145 ± 47 mg/L and 121 ± 45 mg/L respectively, n = 38, p < 0.0001). Conclusions: A GP-HPLC technique provides LDL-TG levels comparable to those obtained by the UC/precipitation method.

Original language	English
Pages (from-to)	114-117
Number of pages	4
Journal	Clinical Biochemistry
Volume	42
Issue number	1-2
DOIs	https://doi.org/10.1016/j.clinbiochem.2008.10.007
Publication status	Published - Jan 2009

Keywords

Cholesterol
HPLC
Lipoprotein
Triglyceride

ASJC Scopus subject areas

Clinical Biochemistry

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10.1016/j.clinbiochem.2008.10.007

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Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method. / Usui, Shinichi; Mizuno, Tetsuya; Okazaki, Mitsuyo et al.
In: Clinical Biochemistry, Vol. 42, No. 1-2, 01.2009, p. 114-117.

Research output: Contribution to journal › Article › peer-review

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abstract = "Objectives: To evaluate the gel permeation high-performance liquid chromatography (GP-HPLC) method for determination of triglyceride (TG) levels in low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Design and methods: The GP-HPLC and the ultracentrifugation(UC)/precipitation methods were used and compared. Results: There was no significant difference in measured levels of LDL-triglyceride between the two methods, but the HDL-triglyceride levels measured by the GP-HPLC technique were significantly higher than the UC/precipitation one (145 ± 47 mg/L and 121 ± 45 mg/L respectively, n = 38, p < 0.0001). Conclusions: A GP-HPLC technique provides LDL-TG levels comparable to those obtained by the UC/precipitation method.",

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author = "Shinichi Usui and Tetsuya Mizuno and Mitsuyo Okazaki and Masakazu Nakamura and Ikunosuke Sakurabayashi",

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AU - Nakamura, Masakazu

AU - Sakurabayashi, Ikunosuke

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KW - Cholesterol

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KW - Lipoprotein

KW - Triglyceride

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Evaluation of a gel-permeation high-performance liquid chromatography for determining triglyceride levels in serum major lipoproteins, compared with the ultracentrifugation/precipitation method

Abstract

Keywords

ASJC Scopus subject areas

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