Evaluation of cytokines in donor site wound fluids

Ichiro Ono, Hironori Gunji, Kazuyoshi Suda, Keiji Iwatsuki, Fumio Kaneko

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)


The aim of the study was to measure concentrations of cytokines in wound fluid from donor sites. A film dressing was applied to the donor sites (mean size 10 × 15 cm) immediately after a split skin graft had been taken. Five days later the film was punctured with a needle and 2-3 ml of the fluid accumulated under the film dressing was withdrawn into a syringe. The fluid was snap frozen and stored immediately at -70°C. The fluid was examined for epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), transforming growth factor (TGF)α TGFβ interleukin (IL)-1α and IL-1β The results showed that the fluid accumulated under the film dressing contained growth factors and cytokines that are thought to promote healing. The fluid was rich in TGFα but contained no EGF or bFGF, which indicates that TGFα plays a major part for promoting local wound healing.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalScandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
Issue number4
Publication statusPublished - 1994
Externally publishedYes


  • Cytokines
  • Donor site
  • Transforming growth factor α
  • Wound healing

ASJC Scopus subject areas

  • Medicine(all)


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