Evaluation of the Imaging Process for a Novel Subtraction Method Using Apparent Diffusion Coefficient Values

Kentaro Hamada, Masahiro Kuroda, Yuuki Yoshimura, Abdullah Khasawneh, Majd Barham, Nouha Tekiki, Irfan Sugianto, Babatunde O. Bamgbose, Kohei Konishi, Kohei Sugimoto, Hinata Ishizaka, Akira Kurozumi, Toshi Matsushita, Seiichiro Ohno, Susumu Kanazawa, Junichi Asaumi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Diffusion-weighted imaging may be used to obtain the apparent diffusion coefficient (ADC), which aids the diagnosis of cerebral infarction and tumors. An ADC reflects elements of free diffusion. Diffusion kurtosis imaging (DKI) has attracted attention as a restricted diffusion imaging technique. The ADC subtraction method (ASM) was developed to visualize restricted diffusion with high resolution by using two ADC maps taken with different diffusion times. We conducted the present study to provide a bridge between the reported basic ASM research and clinical research. We developed new imaging software for clinical use and evaluated its performance herein. This software performs the imaging process automatically and continuously at the pixel level, using ImageJ software. The new software uses a macro or a plugin which is compatible with various operating systems via a Java Virtual Machine. We tested the new imaging software's performance by using a Jurkat cell bio-phantom, and the statistical evaluation of the performance clarified that the ASM values of 99.98% of the pixels in the bio-phantom and physiological saline were calculated accurately (p < 0.001). The new software may serve as a useful tool for future clinical applications and restricted diffusion imaging research.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalActa medica Okayama
Issue number2
Publication statusPublished - 2021


  • ADC subtraction method
  • ImageJ
  • apparent diffusion coefficient
  • plugin
  • restricted diffusion

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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