@inbook{74217298c46e4583a01f1e9c9b0b6989,
title = "Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance",
abstract = "CCN proteins are known to bind to various growth factors, cytokines, and membrane proteins. Since these bindings are closely involved in the function of CCN proteins, the analysis of the binding partners is the first step toward understanding the mechanisms of actions of CCN proteins. This chapter describes two approaches used for such analyses: a solid-phase binding assay, which is suitable for confirming the binding easily because of its simplicity and cost advantage, and a surface plasmon resonance assay, which can determine the binding affinities between CCN proteins and their partners.",
keywords = "binding proteins, CCN2, peptide, solid-phase binding assay, SPR",
author = "Eriko Aoyama and Masaharu Takigawa",
note = "Funding Information: We thank Yoshiko Miyake for her secretarial assistance. This work was supported by the programs JSPS KAKENHI Grants-in-aid for Scientific Research (C), No. 19K10053 (to EA) and (B) No. #JP19H03817 (to MT), and Challenging Research (Pioneering) #JP20K20611 (to MT) from the Japan Society for the Promotion of Sciences, Japan. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2744-0_7",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "77--86",
booktitle = "Methods in Molecular Biology",
}