TY - JOUR
T1 - Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum
AU - Wang, Luan
AU - He, Hao
AU - Wang, Shuangchao
AU - Chen, Xiaoguang
AU - Qiu, Dewen
AU - Kondo, Hideki
AU - Guo, Lihua
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 31171818 ). This research was also supported in part by Grants-in-Aid for Scientific Research (C) and on Innovative Areas from the Japanese Ministry of Education, Culture, Sports, Sciences and Technology ( MEXT ) ( KAKENHI Grant numbers 15K07312 , 16H06436 , 16H06429 and 16K21723 ).
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/5
Y1 - 2018/5
N2 - Here we describe a novel (−)ssRNA mycovirus, Fusarium graminearum negative-stranded RNA virus 1 (FgNSRV-1), isolated from Fusarium graminearum strain HN1. The genome of FgNSRV-1 is 9072 nucleotides in length, with five discontinuous but linear ORFs (ORF I-V). Phylogenetic analysis based on entire L polymerase sequences indicated that FgNSRV-1 is related to the (−)ssRNA mycovirus Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1), and other mycoviruses. Our data suggest that FgNSRV-1 can be classified into the family Mymonaviridae, order Mononegavirales. Putative enveloped virion-like structures with filamentous morphology similar to SsNSRV-1 were observed in virion preparation samples. The L proteins of FgNSRV-1, and other fungal mononegaviruses, were found to be related to L protein-like sequences in some fungal genome, supporting the hypothesis that there is coevolution occurring between mycoviruses and fungi. Besides, clearing the virus from the infected host fungus resulted in no discernable phenotypic change.
AB - Here we describe a novel (−)ssRNA mycovirus, Fusarium graminearum negative-stranded RNA virus 1 (FgNSRV-1), isolated from Fusarium graminearum strain HN1. The genome of FgNSRV-1 is 9072 nucleotides in length, with five discontinuous but linear ORFs (ORF I-V). Phylogenetic analysis based on entire L polymerase sequences indicated that FgNSRV-1 is related to the (−)ssRNA mycovirus Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1), and other mycoviruses. Our data suggest that FgNSRV-1 can be classified into the family Mymonaviridae, order Mononegavirales. Putative enveloped virion-like structures with filamentous morphology similar to SsNSRV-1 were observed in virion preparation samples. The L proteins of FgNSRV-1, and other fungal mononegaviruses, were found to be related to L protein-like sequences in some fungal genome, supporting the hypothesis that there is coevolution occurring between mycoviruses and fungi. Besides, clearing the virus from the infected host fungus resulted in no discernable phenotypic change.
KW - Coevolution
KW - FgNSRV-1
KW - Fungus
KW - Fusarium graminearum
KW - Fusarium head blight
KW - Mononegavirus
KW - Mycovirus
KW - Negative-stranded RNA virus
UR - http://www.scopus.com/inward/record.url?scp=85043527537&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85043527537&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2018.03.008
DO - 10.1016/j.virol.2018.03.008
M3 - Article
C2 - 29549785
AN - SCOPUS:85043527537
SN - 0042-6822
VL - 518
SP - 232
EP - 240
JO - Virology
JF - Virology
ER -