TY - JOUR
T1 - Evidence for new β1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera
AU - Kimura, Yoshinobu
AU - Sakamura, Sho
AU - Ushijima, Takayuki
AU - Hama, Yoichiro
AU - Kajiura, Hiroyuki
AU - Fujiyama, Kazuhito
AU - Okihara, Kiyoshi
AU - Hashimoto, Ken
AU - Sugimoto, Hiroyuki
AU - Yamada, Hideo
N1 - Funding Information:
We thank the IS-MS laboratory of Okayama University for MS analysis. This work was supported in part by grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Basic Research C, no. 17580300).
PY - 2007
Y1 - 2007
N2 - In a previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Galβ1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new β1- 3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such β1-3galactosyltransferase activity, which can transfer galactosyl residues with the β1-3 linkage to β1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc 2GlcNAc2Man3-GlcNAc2-PA as acceptor N-glycan, we detected the β1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique β1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.
AB - In a previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Galβ1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new β1- 3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such β1-3galactosyltransferase activity, which can transfer galactosyl residues with the β1-3 linkage to β1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc 2GlcNAc2Man3-GlcNAc2-PA as acceptor N-glycan, we detected the β1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique β1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.
KW - Apis mellifera
KW - N-glycan
KW - Royal jelly glycoprotein
KW - Thomsen-Friedenreich antigen (Galβ1-3GalNAc)
KW - β1-3 galactosyltransferase
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U2 - 10.1271/bbb.70081
DO - 10.1271/bbb.70081
M3 - Article
C2 - 17420569
AN - SCOPUS:34247546701
SN - 0916-8451
VL - 71
SP - 1111
EP - 1114
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 4
ER -