Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1)

Mineo Yamaguchi; Takayuki Sasaki; Mayandi Sivaguru; Yoko Yamamoto; Hiroki Osawa; Ju Ahn Sung; Hideaki Matsumoto

doi:10.1093/pcp/pci083

Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1)

Mineo Yamaguchi, Takayuki Sasaki, Mayandi Sivaguru, Yoko Yamamoto, Hiroki Osawa, Ju Ahn Sung, Hideaki Matsumoto

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat (Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1::green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.

Original language	English
Pages (from-to)	812-816
Number of pages	5
Journal	Plant and Cell Physiology
Volume	46
Issue number	5
DOIs	https://doi.org/10.1093/pcp/pci083
Publication status	Published - May 2005

Keywords

Aluminum stress
Aluminum-activated malate transporter
Green fluorescent protein
Plasma membrane
Triticum aestivum L.

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

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10.1093/pcp/pci083

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title = "Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1)",

abstract = "Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat (Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1::green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.",

keywords = "Aluminum stress, Aluminum-activated malate transporter, Green fluorescent protein, Plasma membrane, Triticum aestivum L.",

author = "Mineo Yamaguchi and Takayuki Sasaki and Mayandi Sivaguru and Yoko Yamamoto and Hiroki Osawa and Sung, {Ju Ahn} and Hideaki Matsumoto",

note = "Funding Information: Anti-PM-ATPase, PPase and BiP were kindly provided by Drs. Hideo Sasakawa, Masayoshi Maeshima and Ikuko Hara-Nishimura, respectively. pTH2 vector was a gift from Dr. Yasuo Niwa. We wish to thank Dr. Mary E. LeNoble for critical reading and correction of the text, and Michiyo Ariyoshi for experimental assistance. This research was supported by Grant-in-Aid for General Research (A) (grant no. 14206008) from the Ministry of Education, Science, Sports and Culture of Japan to H.M. and the Ohara Foundation for Agricultural Sciences",

year = "2005",

month = may,

doi = "10.1093/pcp/pci083",

language = "English",

volume = "46",

pages = "812--816",

journal = "Plant and Cell Physiology",

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publisher = "Oxford University Press",

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AU - Yamaguchi, Mineo

AU - Sasaki, Takayuki

AU - Sivaguru, Mayandi

AU - Yamamoto, Yoko

AU - Osawa, Hiroki

AU - Sung, Ju Ahn

AU - Matsumoto, Hideaki

N1 - Funding Information: Anti-PM-ATPase, PPase and BiP were kindly provided by Drs. Hideo Sasakawa, Masayoshi Maeshima and Ikuko Hara-Nishimura, respectively. pTH2 vector was a gift from Dr. Yasuo Niwa. We wish to thank Dr. Mary E. LeNoble for critical reading and correction of the text, and Michiyo Ariyoshi for experimental assistance. This research was supported by Grant-in-Aid for General Research (A) (grant no. 14206008) from the Ministry of Education, Science, Sports and Culture of Japan to H.M. and the Ohara Foundation for Agricultural Sciences

PY - 2005/5

Y1 - 2005/5

N2 - Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat (Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1::green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.

AB - Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat (Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1::green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.

KW - Aluminum stress

KW - Aluminum-activated malate transporter

KW - Green fluorescent protein

KW - Plasma membrane

KW - Triticum aestivum L.

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Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1)

Abstract

Keywords

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