TY - JOUR
T1 - Evolutionary history of teleost intron-containing and intron-less rhodopsin genes
AU - Fujiyabu, Chihiro
AU - Sato, Keita
AU - Utari, Ni Made Laksmi
AU - Ohuchi, Hideyo
AU - Shichida, Yoshinori
AU - Yamashita, Takahiro
N1 - Funding Information:
We thank Prof. R.S. Molday for the generous gift of a Rho1D4-producing hybridoma. We are also grateful to Dr. E. Nakajima for critical reading of our manuscript. This work was supported in part by Grants-in Aid for Scientific Research of MEXT to K.S. (17K15159), Y.S. (26650119, 16H02515) and T.Y. (16K07437), Grant for Basic Science Research Projects from The Sumitomo Foundation (T.Y.), a grant from Daiichi Sankyo Foundation of Life Science (T.Y.) and CREST, JST JPMJCR1753 (T.Y.). Quite recently, the genomic information of sterlet (Acipenser ruthenus) was deposited at the NCBI genome database (BioProject accession ID: PRJNA491785). Our search of opsin genes in sterlet suggests that the species has only one rhodopsin gene (intron-less gene), which is consistent with our analysis in Siberian sturgeon.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Recent progress in whole genome sequencing has revealed that animals have various kinds of opsin genes for photoreception. Among them, most opsin genes have introns in their coding regions. However, it has been known for a long time that teleost retinas express intron-less rhodopsin genes, which are presumed to have been formed by retroduplication from an ancestral intron-containing rhodopsin gene. In addition, teleosts have an intron-containing rhodopsin gene (exo-rhodopsin) exclusively for pineal photoreception. In this study, to unravel the evolutionary origin of the two teleost rhodopsin genes, we analyzed the rhodopsin genes of non-teleost fishes in the Actinopterygii. The phylogenetic analysis of full-length sequences of bichir, sturgeon and gar rhodopsins revealed that retroduplication of the rhodopsin gene occurred after branching of the bichir lineage. In addition, analysis of the tissue distribution and the molecular properties of bichir, sturgeon and gar rhodopsins showed that the abundant and exclusive expression of intron-containing rhodopsin in the pineal gland and the short lifetime of its meta II intermediate, which leads to optimization for pineal photoreception, were achieved after branching of the gar lineage. Based on these results, we propose a stepwise evolutionary model of teleost intron-containing and intron-less rhodopsin genes.
AB - Recent progress in whole genome sequencing has revealed that animals have various kinds of opsin genes for photoreception. Among them, most opsin genes have introns in their coding regions. However, it has been known for a long time that teleost retinas express intron-less rhodopsin genes, which are presumed to have been formed by retroduplication from an ancestral intron-containing rhodopsin gene. In addition, teleosts have an intron-containing rhodopsin gene (exo-rhodopsin) exclusively for pineal photoreception. In this study, to unravel the evolutionary origin of the two teleost rhodopsin genes, we analyzed the rhodopsin genes of non-teleost fishes in the Actinopterygii. The phylogenetic analysis of full-length sequences of bichir, sturgeon and gar rhodopsins revealed that retroduplication of the rhodopsin gene occurred after branching of the bichir lineage. In addition, analysis of the tissue distribution and the molecular properties of bichir, sturgeon and gar rhodopsins showed that the abundant and exclusive expression of intron-containing rhodopsin in the pineal gland and the short lifetime of its meta II intermediate, which leads to optimization for pineal photoreception, were achieved after branching of the gar lineage. Based on these results, we propose a stepwise evolutionary model of teleost intron-containing and intron-less rhodopsin genes.
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U2 - 10.1038/s41598-019-47028-4
DO - 10.1038/s41598-019-47028-4
M3 - Article
C2 - 31337799
AN - SCOPUS:85069651932
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 10653
ER -