Examination of transpositional activity of nDart1at different stages of rice development

Mika Hayashi-Tsugane, Masahiko Maekawa, Hirokazu Kobayashi, Shigeru Iida, Kazuo Tsugane

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)


    As a useful tool to elucidate gene functions, a rice transposon tagging line has been developed using an active endogenous DNA transposon, nDart1. It was highly desirable to evaluate the transposition timing and frequency of the nDart1elements during rice development to facilitate the generation of an efficient mutant isolation system. Comparison of the detected new insertions at different stages of rice development by transposon display analysis demonstrated that the last heading tiller carry a higher number of nDart1 elements than the main culm. Moreover, it was revealed that the last heading tiller could produce progeny that carried more new insertions of nDart1 elements, mainly as a result of the accumulation of somatic insertions in the parental plant. This report demonstrates that late tillers increase the probability of producing independent mutant lines.

    Original languageEnglish
    Pages (from-to)215-219
    Number of pages5
    JournalGenes and Genetic Systems
    Issue number3
    Publication statusPublished - 2011


    • DNA transposon
    • Gene tagging
    • Tiller
    • Timing of transposition
    • Transposon display

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics


    Dive into the research topics of 'Examination of transpositional activity of nDart1at different stages of rice development'. Together they form a unique fingerprint.

    Cite this