TY - JOUR
T1 - Exercise exerts neuroprotective effects on Parkinson's disease model of rats
AU - Tajiri, Naoki
AU - Yasuhara, Takao
AU - Shingo, Tetsuro
AU - Kondo, Akihiko
AU - Yuan, Wenji
AU - Kadota, Tomohito
AU - Wang, Feifei
AU - Baba, Tanefumi
AU - Tayra, Judith Thomas
AU - Morimoto, Takamasa
AU - Jing, Meng
AU - Kikuchi, Yoichiro
AU - Kuramoto, Satoshi
AU - Agari, Takashi
AU - Miyoshi, Yasuyuki
AU - Fujino, Hidemi
AU - Obata, Futoshi
AU - Takeda, Isao
AU - Furuta, Tomohisa
AU - Date, Isao
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan and by grants for Research from Sanyo Broadcasting Co . We also appreciate efforts of Masakazu Yoshida and Yuki Ebuchi to this study.
PY - 2010/1/15
Y1 - 2010/1/15
N2 - Recent studies demonstrate that rehabilitation ameliorates physical and cognitive impairments of patients with stroke, spinal cord injury, and other neurological diseases and that rehabilitation also has potencies to modulate brain plasticity. Here we examined the effects of compulsive exercise on Parkinson's disease model of rats. Before 6-hydroxydopamine (6-OHDA, 20 μg) lesion into the right striatum of female SD rats, bromodeoxyuridine (BrdU) was injected to label the proliferating cells. Subsequently, at 24 h after the lesion, the rats were forced to run on the treadmill (5 days/week, 30 min/day, 11 m/min). As behavioral evaluations, cylinder test was performed at 1, 2, 3, and 4 weeks and amphetamine-induced rotational test was performed at 2 and 4 weeks with consequent euthanasia for immunohistochemical investigations. The exercise group showed better behavioral recovery in cylinder test and significant decrease in the number of amphetamine-induced rotations, compared to the non-exercise group. Correspondingly, significant preservation of tyrosine hydroxylase (TH)-positive fibers in the striatum and TH-positive neurons in the substantia nigra pars compacta (SNc) was demonstrated, compared to the non-exercise group. Additionally, the number of migrated BrdU- and Doublecortin-positive cells toward the lesioned striatum was increased in the exercise group. Furthermore, brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor increased in the striatum by exercise. The results suggest that exercise exerts neuroprotective effects or enhances the neuronal differentiation in Parkinson's disease model of rats with subsequent improvement in deteriorated motor function.
AB - Recent studies demonstrate that rehabilitation ameliorates physical and cognitive impairments of patients with stroke, spinal cord injury, and other neurological diseases and that rehabilitation also has potencies to modulate brain plasticity. Here we examined the effects of compulsive exercise on Parkinson's disease model of rats. Before 6-hydroxydopamine (6-OHDA, 20 μg) lesion into the right striatum of female SD rats, bromodeoxyuridine (BrdU) was injected to label the proliferating cells. Subsequently, at 24 h after the lesion, the rats were forced to run on the treadmill (5 days/week, 30 min/day, 11 m/min). As behavioral evaluations, cylinder test was performed at 1, 2, 3, and 4 weeks and amphetamine-induced rotational test was performed at 2 and 4 weeks with consequent euthanasia for immunohistochemical investigations. The exercise group showed better behavioral recovery in cylinder test and significant decrease in the number of amphetamine-induced rotations, compared to the non-exercise group. Correspondingly, significant preservation of tyrosine hydroxylase (TH)-positive fibers in the striatum and TH-positive neurons in the substantia nigra pars compacta (SNc) was demonstrated, compared to the non-exercise group. Additionally, the number of migrated BrdU- and Doublecortin-positive cells toward the lesioned striatum was increased in the exercise group. Furthermore, brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor increased in the striatum by exercise. The results suggest that exercise exerts neuroprotective effects or enhances the neuronal differentiation in Parkinson's disease model of rats with subsequent improvement in deteriorated motor function.
KW - Dopamine
KW - Neural stem cell
KW - Neurogenesis
KW - Neurotrophic factors
KW - Rehabilitation
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U2 - 10.1016/j.brainres.2009.10.075
DO - 10.1016/j.brainres.2009.10.075
M3 - Article
C2 - 19900418
AN - SCOPUS:73149120471
SN - 0006-8993
VL - 1310
SP - 200
EP - 207
JO - Brain Research
JF - Brain Research
ER -