Existence of a Novel Hemagglutinin Having No Protease Activity in Vibrio mimicus

Munirul Alam; Shin Ichi Miyoshi; Ikuyo Maruo; Chiemi Ogawa; Sumio Shinoda

doi:10.1111/j.1348-0421.1994.tb01809.x

Existence of a Novel Hemagglutinin Having No Protease Activity in Vibrio mimicus

Munirul Alam, Shin Ichi Miyoshi, Ikuyo Maruo, Chiemi Ogawa, Sumio Shinoda

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known HA/protease. A non-protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA (V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.

Original language	English
Pages (from-to)	467-470
Number of pages	4
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	38
Issue number	6
DOIs	https://doi.org/10.1111/j.1348-0421.1994.tb01809.x
Publication status	Published - 1994

Keywords

HA/protease
Hemagglutinin
Vibrio mimicus

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.1994.tb01809.x

Cite this

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title = "Existence of a Novel Hemagglutinin Having No Protease Activity in Vibrio mimicus",

abstract = "The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known HA/protease. A non-protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA (V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.",

keywords = "HA/protease, Hemagglutinin, Vibrio mimicus",

author = "Munirul Alam and Miyoshi, {Shin Ichi} and Ikuyo Maruo and Chiemi Ogawa and Sumio Shinoda",

year = "1994",

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journal = "Microbiology and Immunology",

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AU - Alam, Munirul

AU - Miyoshi, Shin Ichi

AU - Maruo, Ikuyo

AU - Ogawa, Chiemi

AU - Shinoda, Sumio

PY - 1994

Y1 - 1994

N2 - The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known HA/protease. A non-protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA (V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.

AB - The protease elaborated by Vibrio mimicus is known to possess hemagglutinating ability to chicken erythrocytes, the well-known HA/protease. A non-protease hemagglutinin (HA) with strong agglutinating ability towards rabbit erythrocytes was obtained from 32 hr culture supernatant of a pathogenic environmental strain of V. mimicus. This HA (V. mimicus HA: VMHA) appeared stable at relatively higher temperature and agglutinated the erythrocytes from rabbit, guinea pig and mouse but not the erythrocytes from chicken, bovine, horse and sheep. Simple sugars, metal ions and chelating agents failed to inhibit the activity of VMHA. The activity of VMHA was found to be sensitive to digestion by proteolytic enzymes including HA/protease. These results provide evidence for the existence of novel HA other than HA/protease in V. mimicus.

KW - HA/protease

KW - Hemagglutinin

KW - Vibrio mimicus

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ER -

Existence of a Novel Hemagglutinin Having No Protease Activity in Vibrio mimicus

Abstract

Keywords

ASJC Scopus subject areas

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