Exocytosis of D-aspartate from INS-1E clonal β cells

Masafumi Iharada, Miki Hiasa, Ayumi Kobara, Yoshinori Moriyama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


D-Aspartate is present in the central nervous system and various endocrine organs, and modulates their neuroendocrine function. In islets of Langerhans, α and β cells contain D-aspartate. Here we show that INS-1E clonal β cells contain the highest amount of D-aspartate. Immunohistochemical analysis with specific antibodies against D-aspartate indicated that D-aspartate is co-localized with insulin. Upon the addition of K+, both D-aspartate and insulin are secreted from the cells in a Ca2+- dependent manner. A Ca2+ ionophore, A23187, also triggers the release of D-aspartate and insulin in the presence of Ca2+. Bafilomycin A1, a specific inhibitor of V-ATPase and V-ATPase-linked secondary transport, inhibits the secretion of D-aspartate. These results support the idea that D-aspartate is present in insulin-containing secretory granules and co-secreted with insulin through exocytosis.

Original languageEnglish
Pages (from-to)1329-1331
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Issue number7
Publication statusPublished - Jul 2007


  • D-aspartate
  • Insulin
  • Islet of Langerhans
  • α cell
  • β cell

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science


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