Expression analysis of MIF4GD in the rat testis

Kenzo Okada; Masanori Kimura; Yusuke Moriyama; Michiko Nakai; Kazuhiro Kikuchi; Hiroyuki Kaneko; Tetsuo Kunieda; Tadashi Baba; Junko Noguchi

doi:10.1262/jrd.10-138H

Expression analysis of MIF4GD in the rat testis

Kenzo Okada, Masanori Kimura, Yusuke Moriyama, Michiko Nakai, Kazuhiro Kikuchi, Hiroyuki Kaneko, Tetsuo Kunieda, Tadashi Baba, Junko Noguchi

Graduate School of Environmental and Life Science

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The difference between transcriptional and translational activities of genes is one of the remarkable features in spermatogenesis. In this study, we characterized MIF4GD (MIF4G domain containing), a possible translational regulator, to uncover the function during rat spermatogenesis. Western blot analysis revealed the presence of two forms of MIF4GD with molecular sizes of 25 and 16 kDa in various tissues including the testis. An additional 20-kDa form of MIF4GD was also found exclusively in the testis. These three proteins were abundantly present in pachytene spermatocytes and haploid spermatids. Importantly, the 16-kDa MIF4GD was distinguished from the 25- and 20-kDa proteins by presence in fractions of 40S subunits and ribosomes of spermatogenic cells according to analysis of subcellular localizations. These results suggest that the 16-kDa MIF4GD may function as a translational regulator in spermiogenesis.

Original language	English
Pages (from-to)	256-261
Number of pages	6
Journal	Journal of Reproduction and Development
Volume	57
Issue number	2
DOIs	https://doi.org/10.1262/jrd.10-138H
Publication status	Published - Apr 2011

Keywords

Rat
Spermatogenesis
Translation regulation

ASJC Scopus subject areas

Animal Science and Zoology

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10.1262/jrd.10-138H

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title = "Expression analysis of MIF4GD in the rat testis",

abstract = "The difference between transcriptional and translational activities of genes is one of the remarkable features in spermatogenesis. In this study, we characterized MIF4GD (MIF4G domain containing), a possible translational regulator, to uncover the function during rat spermatogenesis. Western blot analysis revealed the presence of two forms of MIF4GD with molecular sizes of 25 and 16 kDa in various tissues including the testis. An additional 20-kDa form of MIF4GD was also found exclusively in the testis. These three proteins were abundantly present in pachytene spermatocytes and haploid spermatids. Importantly, the 16-kDa MIF4GD was distinguished from the 25- and 20-kDa proteins by presence in fractions of 40S subunits and ribosomes of spermatogenic cells according to analysis of subcellular localizations. These results suggest that the 16-kDa MIF4GD may function as a translational regulator in spermiogenesis.",

keywords = "Rat, Spermatogenesis, Translation regulation",

author = "Kenzo Okada and Masanori Kimura and Yusuke Moriyama and Michiko Nakai and Kazuhiro Kikuchi and Hiroyuki Kaneko and Tetsuo Kunieda and Tadashi Baba and Junko Noguchi",

year = "2011",

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doi = "10.1262/jrd.10-138H",

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T1 - Expression analysis of MIF4GD in the rat testis

AU - Okada, Kenzo

AU - Kimura, Masanori

AU - Moriyama, Yusuke

AU - Nakai, Michiko

AU - Kikuchi, Kazuhiro

AU - Kaneko, Hiroyuki

AU - Kunieda, Tetsuo

AU - Baba, Tadashi

AU - Noguchi, Junko

PY - 2011/4

Y1 - 2011/4

N2 - The difference between transcriptional and translational activities of genes is one of the remarkable features in spermatogenesis. In this study, we characterized MIF4GD (MIF4G domain containing), a possible translational regulator, to uncover the function during rat spermatogenesis. Western blot analysis revealed the presence of two forms of MIF4GD with molecular sizes of 25 and 16 kDa in various tissues including the testis. An additional 20-kDa form of MIF4GD was also found exclusively in the testis. These three proteins were abundantly present in pachytene spermatocytes and haploid spermatids. Importantly, the 16-kDa MIF4GD was distinguished from the 25- and 20-kDa proteins by presence in fractions of 40S subunits and ribosomes of spermatogenic cells according to analysis of subcellular localizations. These results suggest that the 16-kDa MIF4GD may function as a translational regulator in spermiogenesis.

AB - The difference between transcriptional and translational activities of genes is one of the remarkable features in spermatogenesis. In this study, we characterized MIF4GD (MIF4G domain containing), a possible translational regulator, to uncover the function during rat spermatogenesis. Western blot analysis revealed the presence of two forms of MIF4GD with molecular sizes of 25 and 16 kDa in various tissues including the testis. An additional 20-kDa form of MIF4GD was also found exclusively in the testis. These three proteins were abundantly present in pachytene spermatocytes and haploid spermatids. Importantly, the 16-kDa MIF4GD was distinguished from the 25- and 20-kDa proteins by presence in fractions of 40S subunits and ribosomes of spermatogenic cells according to analysis of subcellular localizations. These results suggest that the 16-kDa MIF4GD may function as a translational regulator in spermiogenesis.

KW - Rat

KW - Spermatogenesis

KW - Translation regulation

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IS - 2

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Expression analysis of MIF4GD in the rat testis

Abstract

Keywords

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