Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from arabidopsis and a bacterial gene for β-glucuronidase

Taku Takahashi; Naoto Yabe; Yoshibumi Komeda

Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from arabidopsis and a bacterial gene for β-glucuronidase

Taku Takahashi, Naoto Yabe, Yoshibumi Komeda

Research output: Contribution to journal › Article › peer-review

Abstract

Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

Original language	English
Pages (from-to)	161-164
Number of pages	4
Journal	Plant and Cell Physiology
Volume	34
Issue number	1
Publication status	Published - Jan 1993
Externally published	Yes

Keywords

Arabidopsis thaliana
CAMP
Heat shock
Saccharomyces cerevisiae
Schizosaccharomyces pombe
β-Glucuronidase

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

Cite this

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title = "Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from arabidopsis and a bacterial gene for β-glucuronidase",

abstract = "Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.",

keywords = "Arabidopsis thaliana, CAMP, Heat shock, Saccharomyces cerevisiae, Schizosaccharomyces pombe, β-Glucuronidase",

author = "Taku Takahashi and Naoto Yabe and Yoshibumi Komeda",

note = "Funding Information: We would like to thank Dr. T. Morishita for gifts of S. cerevisiae strains and skilled technical assistance. We also thank Prof. M. Yamamoto for providing a strain of S.pombe. This work was supported in part by a grant-in-aid from the Ministry of Education, Science and Culture of Japan.",

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AU - Takahashi, Taku

AU - Yabe, Naoto

AU - Komeda, Yoshibumi

N1 - Funding Information: We would like to thank Dr. T. Morishita for gifts of S. cerevisiae strains and skilled technical assistance. We also thank Prof. M. Yamamoto for providing a strain of S.pombe. This work was supported in part by a grant-in-aid from the Ministry of Education, Science and Culture of Japan.

PY - 1993/1

Y1 - 1993/1

N2 - Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

AB - Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

KW - Arabidopsis thaliana

KW - CAMP

KW - Heat shock

KW - Saccharomyces cerevisiae

KW - Schizosaccharomyces pombe

KW - β-Glucuronidase

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JF - Plant and Cell Physiology

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Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from arabidopsis and a bacterial gene for β-glucuronidase

Abstract

Keywords

ASJC Scopus subject areas

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