TY - JOUR
T1 - Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing
AU - Nakata, E.
AU - Nakanishi, T.
AU - Kawai, A.
AU - Asaumi, K.
AU - Yamaai, T.
AU - Asano, M.
AU - Nishida, T.
AU - Mitani, S.
AU - Inoue, H.
AU - Takigawa, M.
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (T.N., A.K., T.N., S.M., M.T.), and grants from the Research for the Future Program of the Japan Society for the Promotion of Science (Biological Tissue Engineering Project), Contract Grant No. JSPS-RFTF98100201; the Naito Foundation (M.T.); the Nakatomi Health Science Foundation (M.T.); the Foundation for Growth Science in Japan (M.T.); and the Sumitomo Foundation (M.T.). The authors thank Dr. Satoshi Kubota, Dr. Takako Hattori, Dr. Seiji Kondo, Dr. Gen Yoshimichi, Dr. Norifumi Moritani, Dr. Eguchi Takanori, and Dr. Kumiko Nawachi for valuable discussions. We also thank Aki Yoshida, Yuki Nonami, and Ayako Shizuku for secretarial assistance.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Localization and expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing in mouse ribs were investigated. In situ hybridization demonstrated that CTGF/Hcs24 mRNA was remarkably expressed, especially in hypertrophic chondrocytes and proliferating chondrocytes, in the regions of regenerating cartilage on days 8 and 14 after fracture. CTGF/Hcs24 mRNA was also expressed in proliferating periosteal cells in the vicinity of the fracture sites on days 2 and 8, and in cells in fibrous tissue around the callus on day 8. Northern blot analysis showed that expression of CTGF/Hcs24 mRNA was 3.9 times higher on day 2 of fracture healing than that on day 0. On day 8, it reached a peak of 8.6 times higher than that on day 0. It then declined to a lower level. Immunostaining showed that CTGF/Hcs24 was localized in hypertrophic chondrocytes and proliferating chondrocytes in the regions of regenerating cartilage, and in active osteoblasts in the regions of intramembranous ossification. Although CTGF/Hcs24 was abundant in the proliferating and differentiating cells (on days 8 and 14), immunostaining decreased as the cells differentiated to form bone (on day 20). CTGF/Hcs24 was also detected in cells in fibrous tissue, vascular endothelial cells in the callus, and periosteal cells around the fracture sites. These results suggest that CTGF/Hcs24 plays some role in fracture healing.
AB - Localization and expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing in mouse ribs were investigated. In situ hybridization demonstrated that CTGF/Hcs24 mRNA was remarkably expressed, especially in hypertrophic chondrocytes and proliferating chondrocytes, in the regions of regenerating cartilage on days 8 and 14 after fracture. CTGF/Hcs24 mRNA was also expressed in proliferating periosteal cells in the vicinity of the fracture sites on days 2 and 8, and in cells in fibrous tissue around the callus on day 8. Northern blot analysis showed that expression of CTGF/Hcs24 mRNA was 3.9 times higher on day 2 of fracture healing than that on day 0. On day 8, it reached a peak of 8.6 times higher than that on day 0. It then declined to a lower level. Immunostaining showed that CTGF/Hcs24 was localized in hypertrophic chondrocytes and proliferating chondrocytes in the regions of regenerating cartilage, and in active osteoblasts in the regions of intramembranous ossification. Although CTGF/Hcs24 was abundant in the proliferating and differentiating cells (on days 8 and 14), immunostaining decreased as the cells differentiated to form bone (on day 20). CTGF/Hcs24 was also detected in cells in fibrous tissue, vascular endothelial cells in the callus, and periosteal cells around the fracture sites. These results suggest that CTGF/Hcs24 plays some role in fracture healing.
KW - Connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24)
KW - Endochondral ossification
KW - Fracture healing
KW - Immunostaining
KW - In situ hybridization
KW - Intramembranous ossification
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U2 - 10.1016/S8756-3282(02)00846-3
DO - 10.1016/S8756-3282(02)00846-3
M3 - Article
C2 - 12398938
AN - SCOPUS:0036795874
SN - 8756-3282
VL - 31
SP - 441
EP - 447
JO - Bone
JF - Bone
IS - 4
ER -
