Expression of functional acetylcholine receptor from cloned cDNAs

Masayoshi Mishina, Tomohiro Kurosaki, Takamasa Tobimatsu, Yuuki Morimoto, Masaharu Noda, Tokuo Yamamoto, Mineko Terao, Jon Lindstrom, Tomoyuki Takahashi, Motoy Kuno, Shosaku Numa

Research output: Contribution to journalArticlepeer-review

273 Citations (Scopus)


The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

Original languageEnglish
Pages (from-to)604-608
Number of pages5
Issue number5952
Publication statusPublished - 1984
Externally publishedYes

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