Expression of functional acetylcholine receptor from cloned cDNAs

Masayoshi Mishina; Tomohiro Kurosaki; Takamasa Tobimatsu; Yuuki Morimoto; Masaharu Noda; Tokuo Yamamoto; Mineko Terao; Jon Lindstrom; Tomoyuki Takahashi; Motoy Kuno; Shosaku Numa

doi:10.1038/307604a0

Expression of functional acetylcholine receptor from cloned cDNAs

Masayoshi Mishina, Tomohiro Kurosaki, Takamasa Tobimatsu, Yuuki Morimoto, Masaharu Noda, Tokuo Yamamoto, Mineko Terao, Jon Lindstrom, Tomoyuki Takahashi, Motoy Kuno, Shosaku Numa

Research output: Contribution to journal › Article › peer-review

273 Citations (Scopus)

Abstract

The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

Original language	English
Pages (from-to)	604-608
Number of pages	5
Journal	Nature
Volume	307
Issue number	5952
DOIs	https://doi.org/10.1038/307604a0
Publication status	Published - 1984
Externally published	Yes

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title = "Expression of functional acetylcholine receptor from cloned cDNAs",

abstract = "The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.",

author = "Masayoshi Mishina and Tomohiro Kurosaki and Takamasa Tobimatsu and Yuuki Morimoto and Masaharu Noda and Tokuo Yamamoto and Mineko Terao and Jon Lindstrom and Tomoyuki Takahashi and Motoy Kuno and Shosaku Numa",

year = "1984",

doi = "10.1038/307604a0",

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T1 - Expression of functional acetylcholine receptor from cloned cDNAs

AU - Mishina, Masayoshi

AU - Kurosaki, Tomohiro

AU - Tobimatsu, Takamasa

AU - Morimoto, Yuuki

AU - Noda, Masaharu

AU - Yamamoto, Tokuo

AU - Terao, Mineko

AU - Lindstrom, Jon

AU - Takahashi, Tomoyuki

AU - Kuno, Motoy

AU - Numa, Shosaku

PY - 1984

Y1 - 1984

N2 - The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

AB - The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

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JO - Nature

JF - Nature

IS - 5952

ER -

Expression of functional acetylcholine receptor from cloned cDNAs

Abstract

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