Expression of heme oxygenase-1 is associated with abortion caused by Brucella abortus infection in pregnant mice

Masato Tachibana; Kenta Watanabe; Yuki Yamasaki; Hiroshi Suzuki; Masahisa Watarai

doi:10.1016/j.micpath.2008.04.002

Expression of heme oxygenase-1 is associated with abortion caused by Brucella abortus infection in pregnant mice

Masato Tachibana, Kenta Watanabe, Yuki Yamasaki, Hiroshi Suzuki, Masahisa Watarai

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Brucella abortus is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant (TG) cells, and the causative agent of brucellosis. In the present study, we found that expression of heme oxygenase-1 (HO-1) in TG cells is correlated with abortion induced by B. abortus infection in pregnant mice. Expression of HO-1 in the placenta was decreased by B. abortus infection and treatment with cobalt-protoporphyrin (Co-PP), which is known to up-regulate HO-1 expression, inhibited abortion due to the bacterial infection. In TG cells, treatment with Co-PP was shown to up-regulate HO-1, whereas its expression was decreased by B. abortus infection. Such down-regulation of HO-1 in the TG cells was enhanced by IFN-γ treatment. HO-1 down-regulation in TG cells due to knockdown or IFN-γ treatment served to induce cell death caused by B. abortus infection. These results suggest that down-regulation of HO-1 in TG cells due to B. abortus infection is an important event in infectious abortion.

Original language	English
Pages (from-to)	105-109
Number of pages	5
Journal	Microbial Pathogenesis
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/j.micpath.2008.04.002
Publication status	Published - Aug 2008
Externally published	Yes

Keywords

Abortion
Brucella abortus
HO-1
Placenta

ASJC Scopus subject areas

Microbiology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micpath.2008.04.002

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title = "Expression of heme oxygenase-1 is associated with abortion caused by Brucella abortus infection in pregnant mice",

abstract = "Brucella abortus is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant (TG) cells, and the causative agent of brucellosis. In the present study, we found that expression of heme oxygenase-1 (HO-1) in TG cells is correlated with abortion induced by B. abortus infection in pregnant mice. Expression of HO-1 in the placenta was decreased by B. abortus infection and treatment with cobalt-protoporphyrin (Co-PP), which is known to up-regulate HO-1 expression, inhibited abortion due to the bacterial infection. In TG cells, treatment with Co-PP was shown to up-regulate HO-1, whereas its expression was decreased by B. abortus infection. Such down-regulation of HO-1 in the TG cells was enhanced by IFN-γ treatment. HO-1 down-regulation in TG cells due to knockdown or IFN-γ treatment served to induce cell death caused by B. abortus infection. These results suggest that down-regulation of HO-1 in TG cells due to B. abortus infection is an important event in infectious abortion.",

keywords = "Abortion, Brucella abortus, HO-1, Placenta",

author = "Masato Tachibana and Kenta Watanabe and Yuki Yamasaki and Hiroshi Suzuki and Masahisa Watarai",

note = "Funding Information: We thank Dr. Alexander Cox for critical reading of the manuscript. This work was supported, in part, by grants from the Program for Promotion of Basic Research Activities for Innovative Bioscience, and from Scientific Research (19041006 and 18790298), Japan Society for the Promotion of Science, and from the Institute for Fermentation, Osaka.",

year = "2008",

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doi = "10.1016/j.micpath.2008.04.002",

language = "English",

volume = "45",

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journal = "Microbial Pathogenesis",

issn = "0882-4010",

publisher = "Academic Press Inc.",

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T1 - Expression of heme oxygenase-1 is associated with abortion caused by Brucella abortus infection in pregnant mice

AU - Tachibana, Masato

AU - Watanabe, Kenta

AU - Yamasaki, Yuki

AU - Suzuki, Hiroshi

AU - Watarai, Masahisa

N1 - Funding Information: We thank Dr. Alexander Cox for critical reading of the manuscript. This work was supported, in part, by grants from the Program for Promotion of Basic Research Activities for Innovative Bioscience, and from Scientific Research (19041006 and 18790298), Japan Society for the Promotion of Science, and from the Institute for Fermentation, Osaka.

PY - 2008/8

Y1 - 2008/8

N2 - Brucella abortus is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant (TG) cells, and the causative agent of brucellosis. In the present study, we found that expression of heme oxygenase-1 (HO-1) in TG cells is correlated with abortion induced by B. abortus infection in pregnant mice. Expression of HO-1 in the placenta was decreased by B. abortus infection and treatment with cobalt-protoporphyrin (Co-PP), which is known to up-regulate HO-1 expression, inhibited abortion due to the bacterial infection. In TG cells, treatment with Co-PP was shown to up-regulate HO-1, whereas its expression was decreased by B. abortus infection. Such down-regulation of HO-1 in the TG cells was enhanced by IFN-γ treatment. HO-1 down-regulation in TG cells due to knockdown or IFN-γ treatment served to induce cell death caused by B. abortus infection. These results suggest that down-regulation of HO-1 in TG cells due to B. abortus infection is an important event in infectious abortion.

AB - Brucella abortus is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant (TG) cells, and the causative agent of brucellosis. In the present study, we found that expression of heme oxygenase-1 (HO-1) in TG cells is correlated with abortion induced by B. abortus infection in pregnant mice. Expression of HO-1 in the placenta was decreased by B. abortus infection and treatment with cobalt-protoporphyrin (Co-PP), which is known to up-regulate HO-1 expression, inhibited abortion due to the bacterial infection. In TG cells, treatment with Co-PP was shown to up-regulate HO-1, whereas its expression was decreased by B. abortus infection. Such down-regulation of HO-1 in the TG cells was enhanced by IFN-γ treatment. HO-1 down-regulation in TG cells due to knockdown or IFN-γ treatment served to induce cell death caused by B. abortus infection. These results suggest that down-regulation of HO-1 in TG cells due to B. abortus infection is an important event in infectious abortion.

KW - Abortion

KW - Brucella abortus

KW - HO-1

KW - Placenta

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Expression of heme oxygenase-1 is associated with abortion caused by Brucella abortus infection in pregnant mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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