Expression of human immunoglobulin E ε chain cDNA in E.coli

Tsutornu Kurokawa; Masaharu Seno; Reiko Sasada; Yoshitaka Ona; Haruo Onda; Koichi Igarashi; Masakazu Kikuchi; Yukio Sugino; Tasuku Honjo

doi:10.1093/nar/11.10.3077

Expression of human immunoglobulin E ε chain cDNA in E.coli

Tsutornu Kurokawa, Masaharu Seno, Reiko Sasada, Yoshitaka Ona, Haruo Onda, Koichi Igarashi, Masakazu Kikuchi, Yukio Sugino, Tasuku Honjo

Research output: Contribution to journal › Article › peer-review

Abstract

Using the cDNA of human 1D6DC; chain, three expression plasmids that code directly the constant portion of the 1D6DC; chain (C1D6DC;l-C 1D6DC;4, C 1D6DC;2-C 1D6DC;C4 and C 1D6DC;3-C 1D6DC;4 domains) were constructed. These 1D6DC; chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human 1D6DC; chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.

Original language	English
Pages (from-to)	3077-3085
Number of pages	9
Journal	Nucleic acids research
Volume	11
Issue number	10
DOIs	https://doi.org/10.1093/nar/11.10.3077
Publication status	Published - May 25 1983
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Expression of human immunoglobulin E ε chain cDNA in E.coli",

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author = "Tsutornu Kurokawa and Masaharu Seno and Reiko Sasada and Yoshitaka Ona and Haruo Onda and Koichi Igarashi and Masakazu Kikuchi and Yukio Sugino and Tasuku Honjo",

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T1 - Expression of human immunoglobulin E ε chain cDNA in E.coli

AU - Kurokawa, Tsutornu

AU - Seno, Masaharu

AU - Sasada, Reiko

AU - Ona, Yoshitaka

AU - Onda, Haruo

AU - Igarashi, Koichi

AU - Kikuchi, Masakazu

AU - Sugino, Yukio

AU - Honjo, Tasuku

PY - 1983/5/25

Y1 - 1983/5/25

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AB - Using the cDNA of human 1D6DC; chain, three expression plasmids that code directly the constant portion of the 1D6DC; chain (C1D6DC;l-C 1D6DC;4, C 1D6DC;2-C 1D6DC;C4 and C 1D6DC;3-C 1D6DC;4 domains) were constructed. These 1D6DC; chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human 1D6DC; chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.

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Expression of human immunoglobulin E ε chain cDNA in E.coli

Abstract

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