TY - JOUR
T1 - Expression of immunoglobulin superfamily members on the lymphatic endothelium of inflamed human small intestine
AU - Sawa, Yoshihiko
AU - Shibata, Ken Ichiro
AU - Braithwaite, Mark W.
AU - Suzuki, Masatsugu
AU - Yoshida, Shigemitsu
N1 - Funding Information:
The authors thank Dr. Hiroshi Shiroto, Department of Surgery I, Hokkaido University School of Medicine for help in procuring human small intestine. This work was supported by Grant-in-Aid for Encouragement of Young Scientists (A) (No. 09771490) and by Grant-in-Aid for Scientific Research (B) (2) (No. 09470389) from the Ministry of Education, Science and Culture, Japan.
PY - 1999/3
Y1 - 1999/3
N2 - Previously, lymphatic endothelium of human tissue has been shown to express only platelet-endothelial cell adhesion molecule-1 (PECAM-1). In this study we examined the expression of immunoglobulin superfamily members on the lymphatic endothelium of human small intestine while in the presence of inflammatory cytokines. Lymphatic vessels were identified by using a cocktail of IgGs for desmoplakin I and II while the presence of inflammatory cytokines was determined by the expression of major histocompatibility complex (MHC) class II in the venules. As a result, lymphatic vessels in the tissue with venules expressing MHC class II expressed PECAM-1, intercellular adhesion molecule (ICAM)-1, ICAM-3, and vascular cell adhesion molecule-1 (VCAM-1). The expression of ICAM-3 and VCAM-1 was significantly stronger in lymphatic vessels than in blood vessels. The results suggest that inflamed lymphatic endothelium may allow more lymphocyte subpopulations to adhere to the endothelium than non-inflamed lymphatic endothelium, due to the expression of multiple adhesion molecules playing a role.
AB - Previously, lymphatic endothelium of human tissue has been shown to express only platelet-endothelial cell adhesion molecule-1 (PECAM-1). In this study we examined the expression of immunoglobulin superfamily members on the lymphatic endothelium of human small intestine while in the presence of inflammatory cytokines. Lymphatic vessels were identified by using a cocktail of IgGs for desmoplakin I and II while the presence of inflammatory cytokines was determined by the expression of major histocompatibility complex (MHC) class II in the venules. As a result, lymphatic vessels in the tissue with venules expressing MHC class II expressed PECAM-1, intercellular adhesion molecule (ICAM)-1, ICAM-3, and vascular cell adhesion molecule-1 (VCAM-1). The expression of ICAM-3 and VCAM-1 was significantly stronger in lymphatic vessels than in blood vessels. The results suggest that inflamed lymphatic endothelium may allow more lymphocyte subpopulations to adhere to the endothelium than non-inflamed lymphatic endothelium, due to the expression of multiple adhesion molecules playing a role.
KW - Immunoglobulin superfamily
KW - Lymphatic endothelium
KW - MHC class II
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U2 - 10.1006/mvre.1998.2132
DO - 10.1006/mvre.1998.2132
M3 - Article
C2 - 10049658
AN - SCOPUS:0032809042
SN - 0026-2862
VL - 57
SP - 100
EP - 106
JO - Microvascular Research
JF - Microvascular Research
IS - 2
ER -