Expression of neurocan after transient middle cerebral artery occlusion in adult rat brain

Kentaro Deguchi, Mikiro Takaishi, Takeshi Hayashi, Atsuhiko Oohira, Shoko Nagotani, Feng Li, Guang Jin, Isao Nagano, Mikio Shoji, Masahiro Miyazaki, Koji Abe, Nam Ho Huh

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43 Citations (Scopus)


Neurocan is one of the major chondroitin sulfate proteoglycans in the nervous tissues. The expression and proteolytic cleavage of neurocan are developmentally regulated in the normal rat brain, and the full-length neurocan is detected in juvenile brains but not in normal adult brains. Recently, some studies showed that the full-length neurocan was detectable even in the adult brain when it was exposed to mechanical incision or epileptic stimulation. In the present study, we demonstrated by Western blot analysis that the full-length neurocan transiently appeared in the peri-ischemic region of transient middle cerebral artery occlusion (tMCAO) in adult rat with a peak level at 4 days after tMCAO. Immunohistochemical analysis showed that a clear positive signal of neurocan was observed 4 days after tMCAO in the peri-ischemic region of cerebral cortex and caudate, where cells strongly positive in GFAP expression were also distributed. These results indicate that accumulation of the full-length neurocan produced by reactive astrocytes may be one of the processes for tissue repair and reconstruction of neural networks after focal brain ischemia as well.

Original languageEnglish
Pages (from-to)194-199
Number of pages6
JournalBrain Research
Issue number1-2
Publication statusPublished - Mar 10 2005


  • Brain ischemia
  • Chondroitin sulfate proteoglycan
  • Neurocan

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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