TY - JOUR
T1 - Expression patterns of the lysophospholipid receptor genes during mouse early development
AU - Ohuchi, Hideyo
AU - Hamada, Aska
AU - Matsuda, Hironao
AU - Takagi, Akira
AU - Tanaka, Masayuki
AU - Aoki, Junken
AU - Arai, Hiroyuki
AU - Noji, Sumihare
PY - 2008/11
Y1 - 2008/11
N2 - Lysophospholipids (LPs) such as lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are known to mediate various biological responses, including cell proliferation, migration, and differentiation. To better understand the role of these lipids in mammalian early development, we applied whole-mount in situ hybridization techniques to E8.5 to E12.5 mouse embryos. We determined the expression patterns of the following LP receptor genes, which belong to the G protein-coupled receptor (GPCR) family: EDG1 to EDG8 (S1P 1 to S1P5 and LPA1 to LPA3), LPA4 (GPR23 /P2Y9), and LPA5 (GPR92). We found that the S1P/LPA receptor genes exhibit overlapping expression patterns in a variety of organ primordia, including the developing brain and cardiovascular system, presomitic mesoderm and somites, branchial arches, and limb buds. These results suggest that multiple receptor systems for LPA/S1P lysophospholipids may be functioning during organogenesis.
AB - Lysophospholipids (LPs) such as lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are known to mediate various biological responses, including cell proliferation, migration, and differentiation. To better understand the role of these lipids in mammalian early development, we applied whole-mount in situ hybridization techniques to E8.5 to E12.5 mouse embryos. We determined the expression patterns of the following LP receptor genes, which belong to the G protein-coupled receptor (GPCR) family: EDG1 to EDG8 (S1P 1 to S1P5 and LPA1 to LPA3), LPA4 (GPR23 /P2Y9), and LPA5 (GPR92). We found that the S1P/LPA receptor genes exhibit overlapping expression patterns in a variety of organ primordia, including the developing brain and cardiovascular system, presomitic mesoderm and somites, branchial arches, and limb buds. These results suggest that multiple receptor systems for LPA/S1P lysophospholipids may be functioning during organogenesis.
KW - Edg
KW - Endothelial differentiation genes
KW - G protein-coupled receptors
KW - GPR23
KW - GPR92
KW - Gna13
KW - LPA
KW - Lysophosphatidic acid
KW - S1P
KW - Sphingosine-1-phosphate
UR - http://www.scopus.com/inward/record.url?scp=55549114728&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55549114728&partnerID=8YFLogxK
U2 - 10.1002/dvdy.21736
DO - 10.1002/dvdy.21736
M3 - Article
C2 - 18924241
AN - SCOPUS:55549114728
SN - 1058-8388
VL - 237
SP - 3280
EP - 3294
JO - Developmental Dynamics
JF - Developmental Dynamics
IS - 11
ER -