TY - JOUR
T1 - Expression profiles of BDNF splice variants in cultured DRG neurons stimulated with NGF
AU - Matsuoka, Yoshikazu
AU - Yokoyama, Masataka
AU - Kobayashi, Hiroyuki
AU - Omori, Megumi
AU - Itano, Yoshitaro
AU - Morita, Kiyoshi
AU - Mori, Hiroki
AU - Nakanishi, Tohru
PY - 2007/10/26
Y1 - 2007/10/26
N2 - Expression of brain-derived neurotrophic factor (BDNF) mRNA is increased in the dorsal root ganglion (DRG) in response to peripheral inflammation. Nerve growth factor (NGF) from inflammatory tissue is thought to induce expression of BDNF. Recently, it was reported that the BDNF gene has eight non-coding exons that are transcribed independently into several splice variants. Expression of these splice variants in DRG neurons stimulated with NGF has not been studied. We examined changes in expression of BDNF splice variants in a rat model of peripheral inflammation and in cultured DRG neurons exposed to NGF. Total BDNF mRNA was increased by inflammation in vivo and by NGF in vitro. Among all splice variants, exon 1-9 showed the greatest increase in expression in both experiments. Our results indicate that exon 1-9 contributes to changes in total BDNF levels and may play an important role in the acute response of DRG to NGF.
AB - Expression of brain-derived neurotrophic factor (BDNF) mRNA is increased in the dorsal root ganglion (DRG) in response to peripheral inflammation. Nerve growth factor (NGF) from inflammatory tissue is thought to induce expression of BDNF. Recently, it was reported that the BDNF gene has eight non-coding exons that are transcribed independently into several splice variants. Expression of these splice variants in DRG neurons stimulated with NGF has not been studied. We examined changes in expression of BDNF splice variants in a rat model of peripheral inflammation and in cultured DRG neurons exposed to NGF. Total BDNF mRNA was increased by inflammation in vivo and by NGF in vitro. Among all splice variants, exon 1-9 showed the greatest increase in expression in both experiments. Our results indicate that exon 1-9 contributes to changes in total BDNF levels and may play an important role in the acute response of DRG to NGF.
KW - Brain-derived neurotrophic factor (BDNF)
KW - Dorsal root ganglion (DRG)
KW - Inflammatory pain
KW - Nerve growth factor (NGF)
KW - Splice variants
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U2 - 10.1016/j.bbrc.2007.08.022
DO - 10.1016/j.bbrc.2007.08.022
M3 - Article
C2 - 17764660
AN - SCOPUS:34548430455
SN - 0006-291X
VL - 362
SP - 682
EP - 688
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -