Expression profiles of BDNF splice variants in cultured DRG neurons stimulated with NGF

Yoshikazu Matsuoka, Masataka Yokoyama, Hiroyuki Kobayashi, Megumi Omori, Yoshitaro Itano, Kiyoshi Morita, Hiroki Mori, Tohru Nakanishi

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Expression of brain-derived neurotrophic factor (BDNF) mRNA is increased in the dorsal root ganglion (DRG) in response to peripheral inflammation. Nerve growth factor (NGF) from inflammatory tissue is thought to induce expression of BDNF. Recently, it was reported that the BDNF gene has eight non-coding exons that are transcribed independently into several splice variants. Expression of these splice variants in DRG neurons stimulated with NGF has not been studied. We examined changes in expression of BDNF splice variants in a rat model of peripheral inflammation and in cultured DRG neurons exposed to NGF. Total BDNF mRNA was increased by inflammation in vivo and by NGF in vitro. Among all splice variants, exon 1-9 showed the greatest increase in expression in both experiments. Our results indicate that exon 1-9 contributes to changes in total BDNF levels and may play an important role in the acute response of DRG to NGF.

Original languageEnglish
Pages (from-to)682-688
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Oct 26 2007


  • Brain-derived neurotrophic factor (BDNF)
  • Dorsal root ganglion (DRG)
  • Inflammatory pain
  • Nerve growth factor (NGF)
  • Splice variants

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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