Extraction of DNA by reversed micelles

Masahiro Goto; Tsutomu Ono; Akihiko Horiuchi; Shintaro Furusaki

doi:10.1252/jcej.32.123

Extraction of DNA by reversed micelles

Masahiro Goto, Tsutomu Ono, Akihiko Horiuchi, Shintaro Furusaki

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Deoxyribonucleotide (DNA) from salmon testes was successfully extracted by reversed micelles that are formed by a cationic surfactant in isooctane. The degree of extraction of DNA was strongly influenced by the type of surfactant in the organic solvent and ionic strength in the material aqueous phase. The driving force of the DNA transfer was electrostatic interaction between cationic surfactants and negatively charged DNA. Cationic surfactants possessing two long-alkyl chains are one of the best candidates for the successful transfer of DNA from an aqueous solution to a reversed micellar solution.

Original language	English
Pages (from-to)	123-125
Number of pages	3
Journal	JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Volume	32
Issue number	1
DOIs	https://doi.org/10.1252/jcej.32.123
Publication status	Published - 1999
Externally published	Yes

Keywords

Biotechnology
Molecular Assembly
Separation
Surfactant

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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10.1252/jcej.32.123

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AU - Goto, Masahiro

AU - Ono, Tsutomu

AU - Horiuchi, Akihiko

AU - Furusaki, Shintaro

PY - 1999

Y1 - 1999

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AB - Deoxyribonucleotide (DNA) from salmon testes was successfully extracted by reversed micelles that are formed by a cationic surfactant in isooctane. The degree of extraction of DNA was strongly influenced by the type of surfactant in the organic solvent and ionic strength in the material aqueous phase. The driving force of the DNA transfer was electrostatic interaction between cationic surfactants and negatively charged DNA. Cationic surfactants possessing two long-alkyl chains are one of the best candidates for the successful transfer of DNA from an aqueous solution to a reversed micellar solution.

KW - Biotechnology

KW - Molecular Assembly

KW - Separation

KW - Surfactant

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Extraction of DNA by reversed micelles

Abstract

Keywords

ASJC Scopus subject areas

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