Fabrication of reconfigurable protein matrices by cracking

Xiaoyue Zhu; Kristen L. Mills; Portia R. Peters; Joong Hwan Bahng; Elizabeth Ho Liu; Jeongsup Shim; Keiji Naruse; Marie E. Csete; M. D. Thouless; Shuichi Takayama

doi:10.1038/nmat1365

Fabrication of reconfigurable protein matrices by cracking

Xiaoyue Zhu, Kristen L. Mills, Portia R. Peters, Joong Hwan Bahng, Elizabeth Ho Liu, Jeongsup Shim, Keiji Naruse, Marie E. Csete, M. D. Thouless, Shuichi Takayama

Research output: Contribution to journal › Article › peer-review

119 Citations (Scopus)

Abstract

The fabrication of substrates containing arrays of cracks in which cell-adhesive proteins are selectively adsorbed was described. The widths of the cracks were similar in size to individual adhesion complexes and can be modulated by adjusting the mechanical strain applied to the substrate. It was observed that morphology of cells can be reversibly manipulated multiple times through in situ adjustment of crack widths and hence the amount of the cell-adhesive proteins accessible to the cell. The results show that these substrates provide a new tool for assessing cellular responses associated with exposure to matrix proteins.

Original language	English
Pages (from-to)	403-406
Number of pages	4
Journal	Nature Materials
Volume	4
Issue number	5
DOIs	https://doi.org/10.1038/nmat1365
Publication status	Published - May 2005
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1038/nmat1365

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@article{5a1f1971b8b24b4ba0c45feabed14bf5,

title = "Fabrication of reconfigurable protein matrices by cracking",

abstract = "The fabrication of substrates containing arrays of cracks in which cell-adhesive proteins are selectively adsorbed was described. The widths of the cracks were similar in size to individual adhesion complexes and can be modulated by adjusting the mechanical strain applied to the substrate. It was observed that morphology of cells can be reversibly manipulated multiple times through in situ adjustment of crack widths and hence the amount of the cell-adhesive proteins accessible to the cell. The results show that these substrates provide a new tool for assessing cellular responses associated with exposure to matrix proteins.",

author = "Xiaoyue Zhu and Mills, {Kristen L.} and Peters, {Portia R.} and Bahng, {Joong Hwan} and Liu, {Elizabeth Ho} and Jeongsup Shim and Keiji Naruse and Csete, {Marie E.} and Thouless, {M. D.} and Shuichi Takayama",

note = "Funding Information: We thank J. F. Mansfield and C. Wauchope for AFM assistance, S.-H. Chiang for the C2C12 cells, C. Chen for helpful discussions, D. P. Brereton for reviewing the article, BASF Corporation for kindly providing the PLURONIC F108 surfactant, and NIH (EB003793-01, PO1 AG20591), NSF (BES-0238625; DMI-0403603; CTS-0116331) and the NASA BioScience and Engineering Institute (NNC04AA21A) for funding. Correspondence and requests for materials should be addressed to S.T. Supplementary Information accompanies the paper on www.nature.com/naturematerials.",

year = "2005",

month = may,

doi = "10.1038/nmat1365",

language = "English",

volume = "4",

pages = "403--406",

journal = "Nature Materials",

issn = "1476-1122",

publisher = "Nature Publishing Group",

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T1 - Fabrication of reconfigurable protein matrices by cracking

AU - Zhu, Xiaoyue

AU - Mills, Kristen L.

AU - Peters, Portia R.

AU - Bahng, Joong Hwan

AU - Liu, Elizabeth Ho

AU - Shim, Jeongsup

AU - Naruse, Keiji

AU - Csete, Marie E.

AU - Thouless, M. D.

AU - Takayama, Shuichi

N1 - Funding Information: We thank J. F. Mansfield and C. Wauchope for AFM assistance, S.-H. Chiang for the C2C12 cells, C. Chen for helpful discussions, D. P. Brereton for reviewing the article, BASF Corporation for kindly providing the PLURONIC F108 surfactant, and NIH (EB003793-01, PO1 AG20591), NSF (BES-0238625; DMI-0403603; CTS-0116331) and the NASA BioScience and Engineering Institute (NNC04AA21A) for funding. Correspondence and requests for materials should be addressed to S.T. Supplementary Information accompanies the paper on www.nature.com/naturematerials.

PY - 2005/5

Y1 - 2005/5

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AB - The fabrication of substrates containing arrays of cracks in which cell-adhesive proteins are selectively adsorbed was described. The widths of the cracks were similar in size to individual adhesion complexes and can be modulated by adjusting the mechanical strain applied to the substrate. It was observed that morphology of cells can be reversibly manipulated multiple times through in situ adjustment of crack widths and hence the amount of the cell-adhesive proteins accessible to the cell. The results show that these substrates provide a new tool for assessing cellular responses associated with exposure to matrix proteins.

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JO - Nature Materials

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ER -

Fabrication of reconfigurable protein matrices by cracking

Abstract

ASJC Scopus subject areas

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