TY - JOUR
T1 - Female mating receptivity inhibited by injection of male-derived extracts in Callosobruchus chinensis
AU - Yamane, Takashi
AU - Kimura, Yoshinobu
AU - Katsuhara, Maki
AU - Miyatake, Takahisa
N1 - Funding Information:
We thank two anonymous referees for useful comments, Dr. Yoshiyuki Murata for advising the injection method, and Dr. Kenji Matsuura for obtaining facilities for our experiments. This study was supported in part by Research Fellowships for Young Scientists (JSPS) to T.Y., and JSPS Grant-in-Aids for Scientific Research (KAKENHI 16370013, 19370011, and 19657026) from the Japanese Ministry of Education, Science, Sports, and Culture to T.M.
PY - 2008/2
Y1 - 2008/2
N2 - The effects of male-derived extracts on female receptivity to remating were investigated in Callosobruchus chinensis (Coleoptera: Bruchidae). Injection of aqueous extracts of male reproductive tracts into the abdomen of females reduced receptivity. When aqueous extracts of male reproductive tracts were divided to three molecular weight (MW) fractions by ultrafiltration: <3, 3-14, and >14 kDa, the filtrate containing MW substances <3 kDa reduced female receptivity 3 h and 1 day after injection, whereas the fraction containing MW substances >14 kDa inhibited receptivity 2 and 4 days after injection. Finally, male reproductive tract organs were divided into accessory gland, seminal vesicle, and testis. Aqueous extracts of testis reduced receptivity of females on the second day and at 3 h, and aqueous extracts of accessory gland reduced receptivity of females on the second day after injection. On the other hand, aqueous extracts of seminal vesicle did not reduce female receptivity. The results indicate that more than one mechanism may be involved in producing the effects of male-derived substances on female receptivity; low MW male-derived substances, which possibly exist in testis, cause short-term inhibition, while high MW substances, which possibly exist in the accessory gland, inhibit female mating later than low MW substances in C. chinensis.
AB - The effects of male-derived extracts on female receptivity to remating were investigated in Callosobruchus chinensis (Coleoptera: Bruchidae). Injection of aqueous extracts of male reproductive tracts into the abdomen of females reduced receptivity. When aqueous extracts of male reproductive tracts were divided to three molecular weight (MW) fractions by ultrafiltration: <3, 3-14, and >14 kDa, the filtrate containing MW substances <3 kDa reduced female receptivity 3 h and 1 day after injection, whereas the fraction containing MW substances >14 kDa inhibited receptivity 2 and 4 days after injection. Finally, male reproductive tract organs were divided into accessory gland, seminal vesicle, and testis. Aqueous extracts of testis reduced receptivity of females on the second day and at 3 h, and aqueous extracts of accessory gland reduced receptivity of females on the second day after injection. On the other hand, aqueous extracts of seminal vesicle did not reduce female receptivity. The results indicate that more than one mechanism may be involved in producing the effects of male-derived substances on female receptivity; low MW male-derived substances, which possibly exist in testis, cause short-term inhibition, while high MW substances, which possibly exist in the accessory gland, inhibit female mating later than low MW substances in C. chinensis.
KW - Accessory gland
KW - Adzuki bean beetle
KW - Remating inhibition
KW - Seminal fluid
KW - Testis
UR - http://www.scopus.com/inward/record.url?scp=38749124714&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38749124714&partnerID=8YFLogxK
U2 - 10.1016/j.jinsphys.2007.11.009
DO - 10.1016/j.jinsphys.2007.11.009
M3 - Article
C2 - 18177665
AN - SCOPUS:38749124714
SN - 0022-1910
VL - 54
SP - 501
EP - 507
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
IS - 2
ER -