TY - JOUR
T1 - FGF10 is a mesenchymally derived stimulator for epidermal development in the chick embryonic skin
AU - Tao, Hirotaka
AU - Yoshimoto, Yasuko
AU - Yoshioka, Hidefumi
AU - Nohno, Tsutomu
AU - Noji, Sumihare
AU - Ohuchi, Hideyo
N1 - Funding Information:
We are indebted to Jonathan Cooke and Frank McKeon for their generous gifts of reagents. This study was partly supported by grants from the Japanese Ministry of Education, Culture, Sports, Science and Technology to S.N. and H.O.
PY - 2002
Y1 - 2002
N2 - The development of avian cutaneous appendages, feathers and scales, is known to arise from the epithelial-mesenchymal interaction. Here we show that FGF10 is associated with this developmental process as an early signal from mesenchymal cells underlying nascent cutaneous placodes. Expression of Fgf10 was detected in the mesenchymal cells underneath the developing placodes. Forced expression of Fgf10 in the femoral skin suppressed expression of Shh and a zinc finger gene snail-related (cSnR), while induced expression of Bmp2 in the interbud region, resulting in thickening of the epidermal layer. Furthermore, forced expression of Fgf10 in the foot skin caused marked ingrowings of the epidermis. The cells in the epidermal ingrowings expressed β-catenin, proliferating cell nuclear antigen, and an epidermal stem cell marker p63. These results support the idea that FGF10 is a mesenchymally derived stimulator of epidermal development through crosstalk with bone morphogenetic protein (BMP), β-catenin, and other signaling pathways.
AB - The development of avian cutaneous appendages, feathers and scales, is known to arise from the epithelial-mesenchymal interaction. Here we show that FGF10 is associated with this developmental process as an early signal from mesenchymal cells underlying nascent cutaneous placodes. Expression of Fgf10 was detected in the mesenchymal cells underneath the developing placodes. Forced expression of Fgf10 in the femoral skin suppressed expression of Shh and a zinc finger gene snail-related (cSnR), while induced expression of Bmp2 in the interbud region, resulting in thickening of the epidermal layer. Furthermore, forced expression of Fgf10 in the foot skin caused marked ingrowings of the epidermis. The cells in the epidermal ingrowings expressed β-catenin, proliferating cell nuclear antigen, and an epidermal stem cell marker p63. These results support the idea that FGF10 is a mesenchymally derived stimulator of epidermal development through crosstalk with bone morphogenetic protein (BMP), β-catenin, and other signaling pathways.
KW - Bone morphogenetic protein
KW - Chick embryo
KW - Cutaneous appendage formation
KW - Feather
KW - Fibroblast growth factor 10
KW - Mesenchymal factor
KW - Scale
KW - p63
KW - β-catenin
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U2 - 10.1016/S0925-4773(02)00131-4
DO - 10.1016/S0925-4773(02)00131-4
M3 - Article
C2 - 12128204
AN - SCOPUS:0035996955
SN - 0925-4773
VL - 116
SP - 39
EP - 49
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 1-2
ER -