TY - JOUR
T1 - Fgf10 is essential for limb and lung formation
AU - Sekine, Keisuke
AU - Ohuchi, Hideyo
AU - Fujiwara, Masanori
AU - Yamasaki, Masahiro
AU - Yoshizawa, Tatsuya
AU - Sato, Takashi
AU - Yagishita, Naoko
AU - Matsui, Daisuke
AU - Koga, Yoshihiko
AU - Itoh, Nobuyuki
AU - Kato, Shigeaki
N1 - Funding Information:
We thank B.L.M. Hogan for critical reading and helpful discussion throughout this study; S. Noji and S. Bellusci for helpful discussion and advise; R. Yu for critical reading; V.E. Papaioannou, A. Tanaka, A. McMahon, T. Nohno and R.L. Johnson for cDNA probes; and S. Kume, Y. Yogiashi, K. Arioka, T. Kawakami and K. Takeyama for technical assistance. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Sports, Science and Culture of Japan (S.K.).
PY - 1999/1
Y1 - 1999/1
N2 - The interactions between fibroblast growth factors (FGF) and their receptors have important roles in mediating mesenchymal-epithelial cell interactions during embryogenesis. In particular, Fgf10 is predicted to function as a regulator of brain, lung and limb development on the basis of its spatiotemporal expression pattern in the developing embryo. To define the role of Fgf10, we generated Fgf10-deficient mice. Fgf10(-/-) mice died at birth due to the lack of lung development. Trachea was formed, but subsequent pulmonary branching morphogenesis was disrupted. In addition, mutant mice had complete truncation of the fore- and hindlimbs. In Fgf10(-/-) embryos, limb bud formation was initiated but outgrowth of the limb buds did not occur; however, formation of the clavicles was not affected. Analysis of the expression of marker genes in the mutant limb buds indicated that the apical ectodermal ridge (AER) and the zone of polarizing activity (ZPA) did not form. Thus, we show here that Fgf10 serves as an essential regulator of lung and limb formation.
AB - The interactions between fibroblast growth factors (FGF) and their receptors have important roles in mediating mesenchymal-epithelial cell interactions during embryogenesis. In particular, Fgf10 is predicted to function as a regulator of brain, lung and limb development on the basis of its spatiotemporal expression pattern in the developing embryo. To define the role of Fgf10, we generated Fgf10-deficient mice. Fgf10(-/-) mice died at birth due to the lack of lung development. Trachea was formed, but subsequent pulmonary branching morphogenesis was disrupted. In addition, mutant mice had complete truncation of the fore- and hindlimbs. In Fgf10(-/-) embryos, limb bud formation was initiated but outgrowth of the limb buds did not occur; however, formation of the clavicles was not affected. Analysis of the expression of marker genes in the mutant limb buds indicated that the apical ectodermal ridge (AER) and the zone of polarizing activity (ZPA) did not form. Thus, we show here that Fgf10 serves as an essential regulator of lung and limb formation.
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U2 - 10.1038/5096
DO - 10.1038/5096
M3 - Article
C2 - 9916808
AN - SCOPUS:0032947346
SN - 1061-4036
VL - 21
SP - 138
EP - 141
JO - Nature Genetics
JF - Nature Genetics
IS - 1
ER -