Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

Naoki Kinto, Masahiro Iwamoto, Motomi Enomoto-Iwamoto, Sumihare Noji, Hideyo Ohuchi, Hidefumi Yoshioka, Hiroko Kataoka, Yasuhiro Wada, Gao Yuhao, Hideaki E. Takahashi, Shusaku Yoshiki, Akira Yamaguchi

Research output: Contribution to journalArticlepeer-review

87 Citations (Scopus)


We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.

Original languageEnglish
Pages (from-to)319-323
Number of pages5
JournalFEBS Letters
Issue number2-3
Publication statusPublished - Mar 10 1997
Externally publishedYes


  • Bone formation
  • Osteoblast differentiation
  • Sonic hedgehog

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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