Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

Naoki Kinto; Masahiro Iwamoto; Motomi Enomoto-Iwamoto; Sumihare Noji; Hideyo Ohuchi; Hidefumi Yoshioka; Hiroko Kataoka; Yasuhiro Wada; Gao Yuhao; Hideaki E. Takahashi; Shusaku Yoshiki; Akira Yamaguchi

doi:10.1016/S0014-5793(97)00014-8

Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

Naoki Kinto, Masahiro Iwamoto, Motomi Enomoto-Iwamoto, Sumihare Noji, Hideyo Ohuchi, Hidefumi Yoshioka, Hiroko Kataoka, Yasuhiro Wada, Gao Yuhao, Hideaki E. Takahashi, Shusaku Yoshiki, Akira Yamaguchi

Research output: Contribution to journal › Article › peer-review

87 Citations (Scopus)

Abstract

We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.

Original language	English
Pages (from-to)	319-323
Number of pages	5
Journal	FEBS Letters
Volume	404
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(97)00014-8
Publication status	Published - Mar 10 1997
Externally published	Yes

Keywords

Bone formation
Osteoblast differentiation
Sonic hedgehog

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(97)00014-8

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title = "Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation",

abstract = "We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.",

keywords = "Bone formation, Osteoblast differentiation, Sonic hedgehog",

author = "Naoki Kinto and Masahiro Iwamoto and Motomi Enomoto-Iwamoto and Sumihare Noji and Hideyo Ohuchi and Hidefumi Yoshioka and Hiroko Kataoka and Yasuhiro Wada and Gao Yuhao and Takahashi, {Hideaki E.} and Shusaku Yoshiki and Akira Yamaguchi",

year = "1997",

month = mar,

day = "10",

doi = "10.1016/S0014-5793(97)00014-8",

language = "English",

volume = "404",

pages = "319--323",

journal = "FEBS Letters",

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number = "2-3",

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T1 - Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

AU - Kinto, Naoki

AU - Iwamoto, Masahiro

AU - Enomoto-Iwamoto, Motomi

AU - Noji, Sumihare

AU - Ohuchi, Hideyo

AU - Yoshioka, Hidefumi

AU - Kataoka, Hiroko

AU - Wada, Yasuhiro

AU - Yuhao, Gao

AU - Takahashi, Hideaki E.

AU - Yoshiki, Shusaku

AU - Yamaguchi, Akira

PY - 1997/3/10

Y1 - 1997/3/10

N2 - We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.

AB - We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.

KW - Bone formation

KW - Osteoblast differentiation

KW - Sonic hedgehog

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DO - 10.1016/S0014-5793(97)00014-8

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AN - SCOPUS:0031042224

SN - 0014-5793

VL - 404

SP - 319

EP - 323

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

Abstract

Keywords

ASJC Scopus subject areas

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