Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells

Yutaka Hisanaga, Kazuki Nakashima, Eichi Tsuruga, Yuka Nakatomi, Yuji Hatakeyama, Hiroyuki Ishikawa, Yoshihiko Sawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


The elastic system fibers comprise oxytalan, elaunin and elastic fibers, which differ in their relative microfibril and elastin content. Human periodontal ligaments (PDL) contain only oxytalan fibers (pure microfibrils) among them. Since fibulin-5 regulates the organization of elastic fibers to link the fibers to cells, we hypothesized that fibulin-5 may contribute to the formation of oxytalan fibers. We used siRNA for fibulin-5 in PDL cell culture to examine the extracellular deposition of fibrillin-1 and -2, which are the major components of microfibrils. Fibulin-5 was labeled on microfibrils positive for fibrillin-1 and -2. Fibulin-5 suppression reduced the level of fibrillin-1 and -2 deposition to 60% of the control level. These results suggest that fibulin-5 may control the formation of oxytalan fibers, and play a role in the homeostasis of oxytalan fibers.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
Issue number5
Publication statusPublished - Oct 30 2009
Externally publishedYes


  • Fibrillin
  • Fibulin-5
  • Microfibrils
  • Oxytalan fiber
  • Periodontal ligaments

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology


Dive into the research topics of 'Fibulin-5 contributes to microfibril assembly in human periodontal ligament cells'. Together they form a unique fingerprint.

Cite this