FK506, a Ca2+/calmodulin-dependent phosphatase inhibitor, inhibits the induction of long-term potentiation in the rat hippocampus

Yun Fei Lu; Yasushi Hayashi; Akiyoshi Moriwaki; Kazuhito Tomizawa; Hideki Matsui

doi:10.1016/0304-3940(96)12384-3

FK506, a Ca²⁺/calmodulin-dependent phosphatase inhibitor, inhibits the induction of long-term potentiation in the rat hippocampus

Yun Fei Lu, Yasushi Hayashi, Akiyoshi Moriwaki, Kazuhito Tomizawa, Hideki Matsui

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The effects of FK506, a Ca²⁺/calmodulin-dependent phosphatase 2B (calcineurin) inhibitor, on the synaptic potentials and long-term potentiation (LTP) were investigated, using extracellular recordings in the CA1 region of adult rat hippocampal slices. Bath application of FK506 (1-50 μM) produced a dose-dependent and reversible inhibition on the field excitatory postsynaptic potentials. FK506 also showed an inhibitory effect on the induction of LTP evoked by theta-burst stimulation. Analyses of the fiber volley and paired-pulse facilitation revealed that FK506-induced effects were based on postsynaptic mechanisms. These results demonstrate that FK506 inhibits both the synaptic transmission and the LTP induction, and suggest that calcineurin plays a promoting role in the LTP processes in the hippocampus of adult rats.

Original language	English
Pages (from-to)	103-106
Number of pages	4
Journal	Neuroscience Letters
Volume	205
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(96)12384-3
Publication status	Published - Feb 23 1996

Keywords

Calcineurin
FK506
Fiber volley
Hippocampus
Long-term potentiation
Paired-pulse facilitation
Protein phosphatase
Synaptic plasticity

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/0304-3940(96)12384-3

Cite this

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title = "FK506, a Ca2+/calmodulin-dependent phosphatase inhibitor, inhibits the induction of long-term potentiation in the rat hippocampus",

abstract = "The effects of FK506, a Ca2+/calmodulin-dependent phosphatase 2B (calcineurin) inhibitor, on the synaptic potentials and long-term potentiation (LTP) were investigated, using extracellular recordings in the CA1 region of adult rat hippocampal slices. Bath application of FK506 (1-50 μM) produced a dose-dependent and reversible inhibition on the field excitatory postsynaptic potentials. FK506 also showed an inhibitory effect on the induction of LTP evoked by theta-burst stimulation. Analyses of the fiber volley and paired-pulse facilitation revealed that FK506-induced effects were based on postsynaptic mechanisms. These results demonstrate that FK506 inhibits both the synaptic transmission and the LTP induction, and suggest that calcineurin plays a promoting role in the LTP processes in the hippocampus of adult rats.",

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T1 - FK506, a Ca2+/calmodulin-dependent phosphatase inhibitor, inhibits the induction of long-term potentiation in the rat hippocampus

AU - Lu, Yun Fei

AU - Hayashi, Yasushi

AU - Moriwaki, Akiyoshi

AU - Tomizawa, Kazuhito

AU - Matsui, Hideki

PY - 1996/2/23

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N2 - The effects of FK506, a Ca2+/calmodulin-dependent phosphatase 2B (calcineurin) inhibitor, on the synaptic potentials and long-term potentiation (LTP) were investigated, using extracellular recordings in the CA1 region of adult rat hippocampal slices. Bath application of FK506 (1-50 μM) produced a dose-dependent and reversible inhibition on the field excitatory postsynaptic potentials. FK506 also showed an inhibitory effect on the induction of LTP evoked by theta-burst stimulation. Analyses of the fiber volley and paired-pulse facilitation revealed that FK506-induced effects were based on postsynaptic mechanisms. These results demonstrate that FK506 inhibits both the synaptic transmission and the LTP induction, and suggest that calcineurin plays a promoting role in the LTP processes in the hippocampus of adult rats.

AB - The effects of FK506, a Ca2+/calmodulin-dependent phosphatase 2B (calcineurin) inhibitor, on the synaptic potentials and long-term potentiation (LTP) were investigated, using extracellular recordings in the CA1 region of adult rat hippocampal slices. Bath application of FK506 (1-50 μM) produced a dose-dependent and reversible inhibition on the field excitatory postsynaptic potentials. FK506 also showed an inhibitory effect on the induction of LTP evoked by theta-burst stimulation. Analyses of the fiber volley and paired-pulse facilitation revealed that FK506-induced effects were based on postsynaptic mechanisms. These results demonstrate that FK506 inhibits both the synaptic transmission and the LTP induction, and suggest that calcineurin plays a promoting role in the LTP processes in the hippocampus of adult rats.

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