Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria

Yuki Ichinose; Fumiko Taguchi; Masanobu Yamamoto; Mayumi Ohnishi-Kameyama; Tatsuo Atsumi; Masako Iwaki; Hiromi Manabe; Mio Kumagai; Quan Thanh Nguyen; Chi Linh Nguyen; Yoshishige Inagaki; Hiroshi Ono; Kazuhiro Chiku; Tadashi Ishii; Mitsuru Yoshida

doi:10.1007/s10327-013-0464-4

Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria

Yuki Ichinose, Fumiko Taguchi, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Tatsuo Atsumi, Masako Iwaki, Hiromi Manabe, Mio Kumagai, Quan Thanh Nguyen, Chi Linh Nguyen, Yoshishige Inagaki, Hiroshi Ono, Kazuhiro Chiku, Tadashi Ishii, Mitsuru Yoshida

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12 Citations (Scopus)

Abstract

Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences, glycostaining, and molecular masses of purified flagellins revealed that flagellins from all phytopathogenic bacteria investigated were glycosylated. Furthermore, the flagellin in a glycosylation-defective mutant of Xanthomonas campestris pv. campestris (Xcc) had a reduced molecular mass, and motility and virulence of the mutant toward host leaves decreased. These results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagellin glycosylation is required for virulence in Xcc.

Original language	English
Pages (from-to)	359-365
Number of pages	7
Journal	Journal of General Plant Pathology
Volume	79
Issue number	5
DOIs	https://doi.org/10.1007/s10327-013-0464-4
Publication status	Published - Sept 2013

Keywords

Flagellin
Glycosylation
Motility
Virulence

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-013-0464-4

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Ichinose, Y., Taguchi, F., Yamamoto, M., Ohnishi-Kameyama, M., Atsumi, T., Iwaki, M., Manabe, H., Kumagai, M., Nguyen, Q. T., Nguyen, C. L., Inagaki, Y., Ono, H., Chiku, K., Ishii, T., & Yoshida, M. (2013). Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria. Journal of General Plant Pathology, 79(5), 359-365. https://doi.org/10.1007/s10327-013-0464-4

Ichinose, Y, Taguchi, F, Yamamoto, M, Ohnishi-Kameyama, M, Atsumi, T, Iwaki, M, Manabe, H, Kumagai, M, Nguyen, QT, Nguyen, CL, Inagaki, Y, Ono, H, Chiku, K, Ishii, T & Yoshida, M 2013, 'Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria', Journal of General Plant Pathology, vol. 79, no. 5, pp. 359-365. https://doi.org/10.1007/s10327-013-0464-4

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abstract = "Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences, glycostaining, and molecular masses of purified flagellins revealed that flagellins from all phytopathogenic bacteria investigated were glycosylated. Furthermore, the flagellin in a glycosylation-defective mutant of Xanthomonas campestris pv. campestris (Xcc) had a reduced molecular mass, and motility and virulence of the mutant toward host leaves decreased. These results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagellin glycosylation is required for virulence in Xcc.",

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doi = "10.1007/s10327-013-0464-4",

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AU - Ichinose, Yuki

AU - Taguchi, Fumiko

AU - Yamamoto, Masanobu

AU - Ohnishi-Kameyama, Mayumi

AU - Atsumi, Tatsuo

AU - Iwaki, Masako

AU - Manabe, Hiromi

AU - Kumagai, Mio

AU - Nguyen, Quan Thanh

AU - Nguyen, Chi Linh

AU - Inagaki, Yoshishige

AU - Ono, Hiroshi

AU - Chiku, Kazuhiro

AU - Ishii, Tadashi

AU - Yoshida, Mitsuru

PY - 2013/9

Y1 - 2013/9

N2 - Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences, glycostaining, and molecular masses of purified flagellins revealed that flagellins from all phytopathogenic bacteria investigated were glycosylated. Furthermore, the flagellin in a glycosylation-defective mutant of Xanthomonas campestris pv. campestris (Xcc) had a reduced molecular mass, and motility and virulence of the mutant toward host leaves decreased. These results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagellin glycosylation is required for virulence in Xcc.

AB - Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences, glycostaining, and molecular masses of purified flagellins revealed that flagellins from all phytopathogenic bacteria investigated were glycosylated. Furthermore, the flagellin in a glycosylation-defective mutant of Xanthomonas campestris pv. campestris (Xcc) had a reduced molecular mass, and motility and virulence of the mutant toward host leaves decreased. These results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagellin glycosylation is required for virulence in Xcc.

KW - Flagellin

KW - Glycosylation

KW - Motility

KW - Virulence

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Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria

Abstract

Keywords

ASJC Scopus subject areas

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