Fluorescent proteins enhance UVC PDT of cancer cells

Masashi Momiyama; Atsushi Suetsugu; Hiroaki Kimura; Hiroyuki Kishimoto; Ryoichi Aki; Akimitsu Yamada; Harumi Sakurada; Takashi Chishima; Michael Bouvet; Natalia N. Bulgakova; Itaru Endo; Robert M. Hoffman

Fluorescent proteins enhance UVC PDT of cancer cells

Masashi Momiyama, Atsushi Suetsugu, Hiroaki Kimura, Hiroyuki Kishimoto, Ryoichi Aki, Akimitsu Yamada, Harumi Sakurada, Takashi Chishima, Michael Bouvet, Natalia N. Bulgakova, Itaru Endo, Robert M. Hoffman

University Hospital

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Cancer cells, with and without fluorescent protein expression, were irradiated with various doses of UVC (100, 400, and 600 J/m²). Dual-color Lewis lung carcinoma cells (LLC) and U87 human glioma cells, expressing GFP in the nucleus and RFP in the cytoplasm and non-colored LLC and U87 cells were cultured in 96-well plates. Eight hours after seeding, the cells were irradiated with the various doses of UVC. The resulting cell number was determined after 24 hours. Compared to non-colored LLC cells, the number of dual-color LLC cells decreased significantly due to UVC irradiation with 100 J/m² (p=0.003). Although there was no significant difference in the number of dual-color and non-colored U87 cells after 100 J/m² UVC irradiation (p=0.852), the number of dual-color U87 cells decreased significantly with respect to non-colored cells due to UVC irradiation with 400 J/m² and 600 J/m² (p=0.011 and p=0.009, respectively). Thus, both dual-color LLC and dual-color U87 cells were more sensitive to UVC light than non-colored LLC and U87 cells. These results suggest that the expression of fluorescent proteins in cancer cells can enhance photodynamic therapy (PDT) using UVC and possibly with other wavelengths of light as well.

Original language	English
Pages (from-to)	4327-4330
Number of pages	4
Journal	Anticancer research
Volume	32
Issue number	10
Publication status	Published - Oct 2012

Keywords

Cell killing
GFP
Lewis lung carcinoma
RFP
U87 glioma
Uv light

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Fluorescent proteins enhance UVC PDT of cancer cells",

abstract = "Cancer cells, with and without fluorescent protein expression, were irradiated with various doses of UVC (100, 400, and 600 J/m2). Dual-color Lewis lung carcinoma cells (LLC) and U87 human glioma cells, expressing GFP in the nucleus and RFP in the cytoplasm and non-colored LLC and U87 cells were cultured in 96-well plates. Eight hours after seeding, the cells were irradiated with the various doses of UVC. The resulting cell number was determined after 24 hours. Compared to non-colored LLC cells, the number of dual-color LLC cells decreased significantly due to UVC irradiation with 100 J/m2 (p=0.003). Although there was no significant difference in the number of dual-color and non-colored U87 cells after 100 J/m2 UVC irradiation (p=0.852), the number of dual-color U87 cells decreased significantly with respect to non-colored cells due to UVC irradiation with 400 J/m2 and 600 J/m2 (p=0.011 and p=0.009, respectively). Thus, both dual-color LLC and dual-color U87 cells were more sensitive to UVC light than non-colored LLC and U87 cells. These results suggest that the expression of fluorescent proteins in cancer cells can enhance photodynamic therapy (PDT) using UVC and possibly with other wavelengths of light as well.",

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author = "Masashi Momiyama and Atsushi Suetsugu and Hiroaki Kimura and Hiroyuki Kishimoto and Ryoichi Aki and Akimitsu Yamada and Harumi Sakurada and Takashi Chishima and Michael Bouvet and Bulgakova, {Natalia N.} and Itaru Endo and Hoffman, {Robert M.}",

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T1 - Fluorescent proteins enhance UVC PDT of cancer cells

AU - Momiyama, Masashi

AU - Suetsugu, Atsushi

AU - Kimura, Hiroaki

AU - Kishimoto, Hiroyuki

AU - Aki, Ryoichi

AU - Yamada, Akimitsu

AU - Sakurada, Harumi

AU - Chishima, Takashi

AU - Bouvet, Michael

AU - Bulgakova, Natalia N.

AU - Endo, Itaru

AU - Hoffman, Robert M.

PY - 2012/10

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N2 - Cancer cells, with and without fluorescent protein expression, were irradiated with various doses of UVC (100, 400, and 600 J/m2). Dual-color Lewis lung carcinoma cells (LLC) and U87 human glioma cells, expressing GFP in the nucleus and RFP in the cytoplasm and non-colored LLC and U87 cells were cultured in 96-well plates. Eight hours after seeding, the cells were irradiated with the various doses of UVC. The resulting cell number was determined after 24 hours. Compared to non-colored LLC cells, the number of dual-color LLC cells decreased significantly due to UVC irradiation with 100 J/m2 (p=0.003). Although there was no significant difference in the number of dual-color and non-colored U87 cells after 100 J/m2 UVC irradiation (p=0.852), the number of dual-color U87 cells decreased significantly with respect to non-colored cells due to UVC irradiation with 400 J/m2 and 600 J/m2 (p=0.011 and p=0.009, respectively). Thus, both dual-color LLC and dual-color U87 cells were more sensitive to UVC light than non-colored LLC and U87 cells. These results suggest that the expression of fluorescent proteins in cancer cells can enhance photodynamic therapy (PDT) using UVC and possibly with other wavelengths of light as well.

AB - Cancer cells, with and without fluorescent protein expression, were irradiated with various doses of UVC (100, 400, and 600 J/m2). Dual-color Lewis lung carcinoma cells (LLC) and U87 human glioma cells, expressing GFP in the nucleus and RFP in the cytoplasm and non-colored LLC and U87 cells were cultured in 96-well plates. Eight hours after seeding, the cells were irradiated with the various doses of UVC. The resulting cell number was determined after 24 hours. Compared to non-colored LLC cells, the number of dual-color LLC cells decreased significantly due to UVC irradiation with 100 J/m2 (p=0.003). Although there was no significant difference in the number of dual-color and non-colored U87 cells after 100 J/m2 UVC irradiation (p=0.852), the number of dual-color U87 cells decreased significantly with respect to non-colored cells due to UVC irradiation with 400 J/m2 and 600 J/m2 (p=0.011 and p=0.009, respectively). Thus, both dual-color LLC and dual-color U87 cells were more sensitive to UVC light than non-colored LLC and U87 cells. These results suggest that the expression of fluorescent proteins in cancer cells can enhance photodynamic therapy (PDT) using UVC and possibly with other wavelengths of light as well.

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KW - GFP

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KW - RFP

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Fluorescent proteins enhance UVC PDT of cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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