TY - JOUR
T1 - Fourier Transform Infrared Analysis of the S-State Cycle of Water Oxidation in the Microcrystals of Photosystem II
AU - Kato, Yuki
AU - Akita, Fusamichi
AU - Nakajima, Yoshiki
AU - Suga, Michihiro
AU - Umena, Yasufumi
AU - Shen, Jian Ren
AU - Noguchi, Takumi
N1 - Funding Information:
This study was supported in part by JSPS KAKENHI grant numbers JP16K17854 (Y.K.), JP16K21181 (F.A.), JP17H05884, JP16H06162 (M.S.), JP16KT0058, JP15H05588 (Y.U.), JP17H06433 (J.-R.S. and T.N.) JP17H0643419, JP24000018 (J.-R.S.), JP17H06435, JP17H03662 (T.N.) an Asahi Glass Foundation (F.A.), a Kato Memorial Bioscience Foundation (F.A.), and PRESTO from JST Grant No. JPMJPR16P1 (F.A.).
Funding Information:
This study was supported in part by JSPS KAKENHI grant numbers JP16K17854 (Y.K.), JP16K21181 (F.A.), JP17H05884, JP16H06162 (M.S.), JP16KT0058, JP15H05588 (Y.U.), JP17H06433 (J.-R.S. and T.N.), JP17H0643419, JP24000018 (J.-R.S.), JP17H06435, JP17H03662 (T.N.), an Asahi Glass Foundation (F.A.), a Kato Memorial Bioscience Foundation (F.A.), and PRESTO from JST Grant No. JPMJPR16P1 (F.A.).
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/5/3
Y1 - 2018/5/3
N2 - Photosynthetic water oxidation is performed in photosystem II (PSII) through a light-driven cycle of intermediates called S states (S0-S4) at the water oxidizing center. Time-resolved serial femtosecond crystallography (SFX) has recently been applied to the microcrystals of PSII to obtain the structural information on these intermediates. However, it remains unanswered whether the reactions efficiently proceed throughout the S-state cycle retaining the native structures of the intermediates in PSII crystals. We investigated the water oxidation reactions in the PSII microcrystals using flash-induced Fourier transform infrared (FTIR) difference spectroscopy. In comparison with the FTIR spectra in solution, it was shown that all of the metastable intermediates in the microcrystals retained their native structures, and the efficiencies of the S-state transitions remained relatively high, although those of the S2 → S3 and S3 → S0 transitions were slightly lowered possibly due to some restriction of water movement in the crystals.
AB - Photosynthetic water oxidation is performed in photosystem II (PSII) through a light-driven cycle of intermediates called S states (S0-S4) at the water oxidizing center. Time-resolved serial femtosecond crystallography (SFX) has recently been applied to the microcrystals of PSII to obtain the structural information on these intermediates. However, it remains unanswered whether the reactions efficiently proceed throughout the S-state cycle retaining the native structures of the intermediates in PSII crystals. We investigated the water oxidation reactions in the PSII microcrystals using flash-induced Fourier transform infrared (FTIR) difference spectroscopy. In comparison with the FTIR spectra in solution, it was shown that all of the metastable intermediates in the microcrystals retained their native structures, and the efficiencies of the S-state transitions remained relatively high, although those of the S2 → S3 and S3 → S0 transitions were slightly lowered possibly due to some restriction of water movement in the crystals.
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U2 - 10.1021/acs.jpclett.8b00638
DO - 10.1021/acs.jpclett.8b00638
M3 - Article
C2 - 29620370
AN - SCOPUS:85046546176
SN - 1948-7185
VL - 9
SP - 2121
EP - 2126
JO - Journal of Physical Chemistry Letters
JF - Journal of Physical Chemistry Letters
IS - 9
ER -