TY - JOUR
T1 - Frequencies of the M918I mutation in the sodium channel of the diamondback moth in China, Thailand and Japan and its association with pyrethroid resistance
AU - Sonoda, Shoji
AU - Shi, Xueyan
AU - Song, Dunlun
AU - Zhang, Youjun
AU - Li, Jianhong
AU - Wu, Gang
AU - Liu, Yong
AU - Li, Ming
AU - Liang, Pei
AU - Wari, David
AU - Matsumura, Masaya
AU - Minakuchi, Chieka
AU - Tanaka, Toshiharu
AU - Miyata, Tadashi
AU - Gao, Xiwu
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (JSPS) (No. 23580072), the JSPS – National Natural Science Foundation of China (NSFC) Joint Research Project, and the Ohara Foundation. The authors thank Y. Narai (Shimane Prefecture, Japan), K. Matsukura (National Agricultural Research Center for Kyushu Okinawa Region), S. Sukontsbhirom (Department of Agriculture, Thailand), and W. Rushtapakonchai (Thep Wantana Chemical Co. Ltd., Thailand) for insect collection.
PY - 2012/2
Y1 - 2012/2
N2 - In addition to the allele frequencies of the L1014F and T929I mutations which are involved in nerve-insensitive resistance to a pyrethroid, those of the M918I mutation were examined using field strains obtained in China, Thailand, and Japan during 2009-2011. Results show that the resistance allele frequencies at the L1014F site were 89-100%, 97-100% and 65-85%, respectively, for strains in China, Thailand, and Japan. The respective allele frequencies at the T929I site were 86-100%, 70-97% and 58-84% for Chinese, Thai, and Japanese strains. With low frequencies up to 27%, M918I was found in Japan and China, but not in Thailand. The strain homozygous for the M918I and L1014F mutations was established and its resistance level to a pyrethroid was examined. The strain lacks a portion of the sodium channel gene corresponding to the 3' portion of exon 18a, intron 18, and the 5' portion of exon 18b. Nevertheless, the strain showed a similar level of resistance to that which was homozygous for the T929I and L1014F mutations.
AB - In addition to the allele frequencies of the L1014F and T929I mutations which are involved in nerve-insensitive resistance to a pyrethroid, those of the M918I mutation were examined using field strains obtained in China, Thailand, and Japan during 2009-2011. Results show that the resistance allele frequencies at the L1014F site were 89-100%, 97-100% and 65-85%, respectively, for strains in China, Thailand, and Japan. The respective allele frequencies at the T929I site were 86-100%, 70-97% and 58-84% for Chinese, Thai, and Japanese strains. With low frequencies up to 27%, M918I was found in Japan and China, but not in Thailand. The strain homozygous for the M918I and L1014F mutations was established and its resistance level to a pyrethroid was examined. The strain lacks a portion of the sodium channel gene corresponding to the 3' portion of exon 18a, intron 18, and the 5' portion of exon 18b. Nevertheless, the strain showed a similar level of resistance to that which was homozygous for the T929I and L1014F mutations.
KW - Diamondback moth
KW - Insecticide resistance
KW - Plutella xylostella
KW - Pyrethroid
KW - Sodium channel
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U2 - 10.1016/j.pestbp.2011.12.005
DO - 10.1016/j.pestbp.2011.12.005
M3 - Article
AN - SCOPUS:84862800820
SN - 0048-3575
VL - 102
SP - 142
EP - 145
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
IS - 2
ER -