TY - JOUR
T1 - FTIR Microspectroscopic Analysis of the Water Oxidation Reaction in a Single Photosystem II Microcrystal
AU - Kato, Yuki
AU - Haniu, Satoshi
AU - Nakajima, Yoshiki
AU - Akita, Fusamichi
AU - Shen, Jian Ren
AU - Noguchi, Takumi
N1 - Funding Information:
This study was supported by JSPS KAKENHI Grants JP19H02674 (to Y.K.), JP19K22396 (to F.A.), JP17H06433 (to J.-R.S. and T.N.), JP17H06434 (to J.-R.S.), and JP17H06435 and JP17H03662 (to T.N.), and by PRESTO from JST Grant JPMJPR16P1 (to F.A.).
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2020/1/9
Y1 - 2020/1/9
N2 - Microcrystals of photosystem II (PSII) have recently been used to investigate the intermediate structures of the water oxidizing complex during water oxidation by serial femtosecond crystallography using X-ray free electron lasers. To clarify the water oxidation mechanism, it is crucial to know whether the reaction proceeds properly in the microcrystals. In this work, we monitored the water oxidation reaction in a single PSII microcrystal using Fourier transform infrared (FTIR) microspectroscopy with the transmission method. Flash-induced micro-FTIR difference spectra of S-state transitions in a PSII microcrystal showed features virtually identical to the corresponding spectra previously obtained using the attenuated total reflection method for multiple microcrystals, representing the reactions near the crystal surface, as well as the spectra in solution. This observation indicates that the reaction processes of water oxidation proceed with relatively high efficiencies retaining native intermediate structures in the entire inside of a PSII microcrystal.
AB - Microcrystals of photosystem II (PSII) have recently been used to investigate the intermediate structures of the water oxidizing complex during water oxidation by serial femtosecond crystallography using X-ray free electron lasers. To clarify the water oxidation mechanism, it is crucial to know whether the reaction proceeds properly in the microcrystals. In this work, we monitored the water oxidation reaction in a single PSII microcrystal using Fourier transform infrared (FTIR) microspectroscopy with the transmission method. Flash-induced micro-FTIR difference spectra of S-state transitions in a PSII microcrystal showed features virtually identical to the corresponding spectra previously obtained using the attenuated total reflection method for multiple microcrystals, representing the reactions near the crystal surface, as well as the spectra in solution. This observation indicates that the reaction processes of water oxidation proceed with relatively high efficiencies retaining native intermediate structures in the entire inside of a PSII microcrystal.
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U2 - 10.1021/acs.jpcb.9b10154
DO - 10.1021/acs.jpcb.9b10154
M3 - Article
C2 - 31825617
AN - SCOPUS:85077435529
SN - 1520-6106
VL - 124
SP - 121
EP - 127
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 1
ER -