Function of β2-adrenergic receptors in chronic localized myalgia

Kenji Maekawa, Takuo Kuboki, Eitoku Inoue, Mami Inoue-Minakuchi, Koji Suzuki, Hirofumi Yatani, Glenn T. Clark

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Aims: To investigate alteration of β2-adrenergic receptor (β2AR) function in chronic localized myalgia subjects by evaluating levels of the β2AR second messenger, cyclic adenosine monophosphate (cAMP), in mononuclear cells after βAR-agonist stimulation. Methods: Eleven chronic localized myalgia subjects and 21 matched healthy controls participated in this study. Peripheral blood (30 cc) was drawn from the subjects' anterocubital vein. Mononuclear cells were isolated from the total blood by using the Ficoll-Hypaque gradient technique. Basal and stimulated intracellular cAMP levels were determined by enzyme immunoassay using a commercially available kit. Aliquots of 5 × 106 cells were incubated with or without stimulation of the βAR-agonist isoproterenol for 5 minutes. Five different concentrations of isoproterenol (10-3M to 10-7M) were utilized. cAMP levels in both groups were tested statistically by a 2-way repeated-measures ANOVA with 2 predictors, group difference and isoproterenol concentration difference. As with isoproterenol stimulation, the cAMP responses to forskolin, which activates adenylyl cyclase directly and produces cAMP, bypassing the cell surface receptors were also measured. Results: The basal cAMP levels in both groups (myalgia: 0.33 ± 0.02 pmol/5 × 106 cells; control: 0.43 ± 0.10 pmol/5 × 106 cells) were almost identical, and isoproterenol-produced cAMP levels increased dose-dependently in both groups. No significant differences in the mean cAMP levels were observed between the groups (P = .909). Significant increases were observed according to the isoproterenol concentration increase (P < .0001). The cAMP responses to forskolin stimulation also showed no significant group difference (P = .971). Conclusion: These results suggest that β2AR function is not different between localized myalgia subjects and healthy individuals.

Original languageEnglish
Pages (from-to)140-144
Number of pages5
JournalJournal of Orofacial Pain
Issue number2
Publication statusPublished - 2003


  • Isoproterenol
  • Localized myalgia
  • Mononuclear cell
  • cAMP
  • β-adrenergic receptor

ASJC Scopus subject areas

  • Dentistry(all)
  • Clinical Neurology
  • Anesthesiology and Pain Medicine


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