TY - JOUR
T1 - Functional characterization of an aluminum (Al)-inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice
AU - Che, Jing
AU - Tsutsui, Tomokazu
AU - Yokosho, Kengo
AU - Yamaji, Naoki
AU - Ma, Jian Feng
N1 - Funding Information:
This work was supported by Grant-in-Aid for Specially Promoted Research (JSPS KAKENHI Grant no. 16H06296 to J.F.M.). We thank Dr Masaki Endo for providing pU6gRNA and pZH_gYSA_MMCas9 for generation of CRISPR/Cas9 lines.
Publisher Copyright:
© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust
PY - 2018/10
Y1 - 2018/10
N2 - High aluminum (Al) tolerance in rice (Oryza sativa) is controlled by a Cys2His2-type zinc finger transcription factor ART1 (Al resistance transcription factor 1). There are five close homologs of ART1 in the rice genome, but the role of these homologs is unknown. We functionally characterized one of the ART1 homologs, ART2, in terms of tissue and spatial expression, subcellular localization, transcriptional activation activity, and phenotypic analysis of the knockout lines. ART2 was localized to the nucleus and showed a transcriptional activation potential in yeast. ART2 was mainly expressed in the roots, but the expression level was much lower than that of ART1. The ART2 expression was rapidly induced by Al in the roots of the wild-type rice, but not in art1 mutant. Knockout of ART2 resulted in increased sensitivity to Al toxicity, but did not alter sensitivity to different pH values. Expression profile analysis by RNA-sequencing showed that ART2 was not involved in activation of genes regulated by ART1; however, four genes seems to be regulated by ART2, which are implicated in Al tolerance. These results indicate that ART1 and ART2 regulate different pathways leading to Al tolerance, and ATR2 plays a supplementary role in Al tolerance in rice.
AB - High aluminum (Al) tolerance in rice (Oryza sativa) is controlled by a Cys2His2-type zinc finger transcription factor ART1 (Al resistance transcription factor 1). There are five close homologs of ART1 in the rice genome, but the role of these homologs is unknown. We functionally characterized one of the ART1 homologs, ART2, in terms of tissue and spatial expression, subcellular localization, transcriptional activation activity, and phenotypic analysis of the knockout lines. ART2 was localized to the nucleus and showed a transcriptional activation potential in yeast. ART2 was mainly expressed in the roots, but the expression level was much lower than that of ART1. The ART2 expression was rapidly induced by Al in the roots of the wild-type rice, but not in art1 mutant. Knockout of ART2 resulted in increased sensitivity to Al toxicity, but did not alter sensitivity to different pH values. Expression profile analysis by RNA-sequencing showed that ART2 was not involved in activation of genes regulated by ART1; however, four genes seems to be regulated by ART2, which are implicated in Al tolerance. These results indicate that ART1 and ART2 regulate different pathways leading to Al tolerance, and ATR2 plays a supplementary role in Al tolerance in rice.
KW - aluminum (Al) tolerance
KW - aluminum resistance transcription factor 2 (ART2)
KW - regulation
KW - rice
KW - transcription factor
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U2 - 10.1111/nph.15252
DO - 10.1111/nph.15252
M3 - Article
C2 - 29888411
AN - SCOPUS:85052510325
SN - 0028-646X
VL - 220
SP - 209
EP - 218
JO - New Phytologist
JF - New Phytologist
IS - 1
ER -