Functional characterization of prostaglandin F receptor in the spinal cord for tactile pain (allodynia)

Tadatoshi Muratani, Mikio Nishizawa, Shinji Matsumura, Tamaki Mabuchi, Kohji Abe, Keiko Shimamoto, Toshiaki Minami, Seiji Ito

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Prostaglandin F (PGF) binds to its receptor (FP) to increase the intracellular-free calcium concentration ([Ca2+]i) by coupling of FP with Gq protein. Spinal intrathecal administration of PGF to mouse induces touch-evoked pain (mechanical allodynia), in which capsaicin-insensitive primary afferent Aβ-fibres and N-methyl-D-aspartate receptor ε4 subunit are involved. FP in the spinal cord, however, was not well characterized. Here, we showed constitutive expression of FP mRNA in mouse spinal cord, and functionally characterized spinal FP-expressing cells which were involved in PGF-induced mechanical allodynia. The method for repetitive administration of oligodeoxyribonucleotides through tubing to conscious mice was established for mechanical allodynia evaluation. We identified an antisense oligodeoxyribonucleotide targeting FP mRNA, causing both disappearance of PGFinduced mechanical allodynia and decrease of FP mRNA. With saline-administered mice, PGF rapidly increased [Ca2+]i of the cells in the deeper layer of the dorsal horn. In contrast, when the FP antisense oligodeoxyribonucleotide was repeatedly administered, the population of PGF-responsive cells in the slices reduced, and PGF-induced [Ca2+]i increase of these cells diminished. These data strongly suggested that, in the dorsal horn of the spinal cord, there are the FP-expressing cells which are involved in PGF-induced mechanical allodynia.

Original languageEnglish
Pages (from-to)374-382
Number of pages9
JournalJournal of Neurochemistry
Issue number2
Publication statusPublished - Jul 2003
Externally publishedYes


  • Antisense oligonucleotide
  • Calcium
  • Mechanical allodynia
  • PGF
  • PGF receptor
  • Spinal cord

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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