TY - JOUR
T1 - Functional domains of template-activating factor-I as a protein phosphatase 2A inhibitor
AU - Saito, Shoko
AU - Miyaji-Yamaguchi, Mary
AU - Shimoyama, Tae
AU - Nagata, Kyosuke
N1 - Funding Information:
This work was supported in part by grants-in-aid from the Ministry of Education, Science, Sports, and Culture of Japan, a grant for the Biodesign Research Program from RIKEN, and a grant from Nagase Science and Technology Foundation.
PY - 1999/6/7
Y1 - 1999/6/7
N2 - Template-Activating Factor-I (TAF-I) α and β, chromatin remodeling factors, were identified as the stimulatory factor for replication of the adenovirus DNA complexed with viral basic core proteins. Recently, two cellular inhibitors for protein phosphatase 2A (PP2A) have been isolated. One of these inhibitors, designated I2(PP2A), is a truncated version of TAF-Iβ. Here, it is shown using recombinant TAF-I proteins that both TAF-Iα and β have the PP2A inhibitor activity. The N-terminal region but not the C-terminal acidic region, the latter of which is essential for the chromatin remodeling activity, is shown to be required for the PP2A inhibitor activity. Roles of TAF-Iα- and β-specific regions, the C-terminal acidic region, and other regions of TAF-I for the PP2A inhibitor activity are also discussed.
AB - Template-Activating Factor-I (TAF-I) α and β, chromatin remodeling factors, were identified as the stimulatory factor for replication of the adenovirus DNA complexed with viral basic core proteins. Recently, two cellular inhibitors for protein phosphatase 2A (PP2A) have been isolated. One of these inhibitors, designated I2(PP2A), is a truncated version of TAF-Iβ. Here, it is shown using recombinant TAF-I proteins that both TAF-Iα and β have the PP2A inhibitor activity. The N-terminal region but not the C-terminal acidic region, the latter of which is essential for the chromatin remodeling activity, is shown to be required for the PP2A inhibitor activity. Roles of TAF-Iα- and β-specific regions, the C-terminal acidic region, and other regions of TAF-I for the PP2A inhibitor activity are also discussed.
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U2 - 10.1006/bbrc.1999.0790
DO - 10.1006/bbrc.1999.0790
M3 - Article
C2 - 10362532
AN - SCOPUS:0033532586
SN - 0006-291X
VL - 259
SP - 471
EP - 475
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -