Functional requirement of CCN2 for intramembranous bone formation in embryonic mice

Harumi Kawaki, Satoshi Kubota, Akiko Suzuki, Tomohiro Yamada, Tatsushi Matsumura, Toshiko Mandai, Mayumi Yao, Takeyasu Maeda, Karen M. Lyons, Masaharu Takigawa

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


CCN2 is best known as a promoter of chondrocyte differentiation among the CCN family members, and Ccn2 null mutant mice display skeletal dysmorphisms. However, little is known concerning the roles of CCN2 during bone formation. We herein present a comparative analysis of wild-type and Ccn2 null mice to investigate the roles of CCN2 in bone development. Multiple histochemical methods were employed to analyze the effects of CCN2 deletion in vivo, and effects of CCN2 on the osteogenic response were evaluated with the isolated and cultured osteoblasts. As a result, we found a drastic reduction of the osteoblastic phenotype in Ccn2 null mutants. Importantly, addition of exogenous CCN2 promoted every step of osteoblast differentiation and rescued the attenuated activities of the Ccn2 null osteoblasts. These results suggest that CCN2 is required not only for the regulation of cartilage and subsequent events, but also for the normal intramembranous bone development.

Original languageEnglish
Pages (from-to)450-456
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Feb 8 2008


  • CCN family
  • Connective tissue growth factor
  • Differentiation
  • Intramembranous bone formation
  • Null mutant
  • Osteoblast

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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