Further Stabilization of Earthworm Serine Protease by Chemical Modification and Immobilization

Nobuyoshi Nakajima, Kohji Ishihara, Manabu Sugimoto, Takashi Nakahara, Hideaki Tsuji

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Earthworm serine protease is more stable and is less affected by organic solvents and detergent than other proteases. However, it is inactivated, probably by autolysis, at 60°C or above under alkaline conditions. Further stabilization was managed by chemical modification of the enzyme with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and phenylglyoxal to protect the activity from the autolytic inactivation. Stabilization was possible also under acidic conditions, in which the stability of the enzyme was rather low, by immobilization with folded sheet mesoporous material. Thus, further stabilization of the enzyme has been achieved by chemical modification or immobilization.

Original languageEnglish
Pages (from-to)2739-2742
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number12
Publication statusPublished - Jan 1 2002


  • Chemical modification
  • Earthworm
  • Immobilization
  • Serine protease
  • Stabilization

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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