TY - JOUR
T1 - Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction
AU - Marutani, Mizuri
AU - Taguchi, Fumiko
AU - Ogawa, Yujiro
AU - Hossain, Md Mijan
AU - Inagaki, Yoshishige
AU - Toyoda, Kazuhiro
AU - Shiraishi, Tomonori
AU - Ichinose, Yuki
N1 - Funding Information:
Acknowledgments We thank Prof. T. Ikeda, University of Utsunomiya, Japan, and Prof. P. Williams, University of Nottingham, UK, for the kind gifts of AHLs and C. violaceum (CV026), respectively. We are grateful to Japan Tobacco Inc., Leaf Tobacco Research Laboratory for providing P. syringae pv. tabaci. This work was supported in part by Grants-in-Aid for ScientiWc Research (S) (No. 15108001) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by the Okayama University COE program “Establishment of Plant Health Science”.
PY - 2008/4
Y1 - 2008/4
N2 - Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-component system-defective mutants, ΔgacA and ΔgacS, and a double mutant, ΔgacAΔgacS, were generated. These mutants produced smaller amounts of N-acyl homoserine lactones required for quorum sensing, had lost swarming motility, and had reduced expression of virulence-related hrp genes and the algT gene required for exopolysaccharide production. The ability of the mutants to cause disease symptoms in their host tobacco plant was remarkably reduced, while they retained the ability to induce hypersensitive reaction (HR) in the nonhost plants. These results indicated that the Gac two-component system of P. syringae pv. tabaci 6605 is indispensable for virulence on the host plant, but not for HR induction in the nonhost plants.
AB - Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-component system-defective mutants, ΔgacA and ΔgacS, and a double mutant, ΔgacAΔgacS, were generated. These mutants produced smaller amounts of N-acyl homoserine lactones required for quorum sensing, had lost swarming motility, and had reduced expression of virulence-related hrp genes and the algT gene required for exopolysaccharide production. The ability of the mutants to cause disease symptoms in their host tobacco plant was remarkably reduced, while they retained the ability to induce hypersensitive reaction (HR) in the nonhost plants. These results indicated that the Gac two-component system of P. syringae pv. tabaci 6605 is indispensable for virulence on the host plant, but not for HR induction in the nonhost plants.
KW - GacA
KW - GacS
KW - Pseudomonas syringae pv. tabaci
KW - Quorum sensing
KW - Two-component system
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U2 - 10.1007/s00438-007-0309-y
DO - 10.1007/s00438-007-0309-y
M3 - Article
C2 - 18080141
AN - SCOPUS:43349103696
SN - 1617-4615
VL - 279
SP - 313
EP - 322
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 4
ER -