Galectin-9 expands immunosuppressive macrophages to ameliorate T-cell-mediated lung inflammation

Tomohiro Arikawa, Naoki Saita, Souichi Oomizu, Masaki Ueno, Akihiro Matsukawa, Shigeki Katoh, Keisuke Kojima, Keiko Nagahara, Minoru Miyake, Akira Yamauchi, Hirotsugu Kohrogi, Mitsuomi Hirashima

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55 Citations (Scopus)


Galectin-9 (Gal-9) plays pivotal roles in the modulation of innate and adaptive immunity to suppress T-cell-mediated autoimmune models. However, it remains unclear if Gal-9 plays a suppressive role for T-cell function in non-autoimmune disease models. We assessed the effects of Gal-9 on experimental hypersensitivity pneumonitis induced by Trichosporon asahii. When Gal-9 was given subcutaneously to C57BL/6 mice at the time of challenge with T. asahii, it significantly suppressed T. asahii-induced lung inflammation, as the levels of IL-1, IL-6, IFN-γ, and IL-17 were significantly reduced in the BALF of Gal-9-treated mice. Moreover, co-culture of anti-CD3-stimulated CD4 T cells with BALF cells harvested from Gal-9-treated mice on day 1 resulted in diminished CD4 T-cell proliferation and decreased levels of IFN-γ and IL-17. CD11b+ Ly-6ChighF4/80+ BALF M/ expanded by Gal-9 were responsible for the suppression.We further found in vitro that Gal-9, only in the presence of T. asahii, expands CD11b+Ly-6C highF4/80+ cells from BM cells, and the cells suppress T-cell proliferation and IFN-γ and IL-17 production. The present results indicate that Gal-9 expands immunosuppressive CD11b+Ly-6C high Mφ to ameliorate Th1/Th17 cell-mediated hypersensitivity pneumonitis.

Original languageEnglish
Pages (from-to)548-558
Number of pages11
JournalEuropean Journal of Immunology
Issue number2
Publication statusPublished - Feb 2010


  • Galectin-9
  • Immune suppression
  • Lung
  • Macrophage
  • Pneumonitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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