Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape

Yoichi Nibe; Tsuyoshi Kimura; Kwangwoo Nam; Shingo Mutsuo; Hidekazu Yoshizawa; Masahiro Okada; Tsutomu Furuzono; Toshiya Fujisato; Akio Kishida

Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape

Yoichi Nibe, Tsuyoshi Kimura, Kwangwoo Nam, Shingo Mutsuo, Hidekazu Yoshizawa, Masahiro Okada, Tsutomu Furuzono, Toshiya Fujisato, Akio Kishida

Research output: Contribution to conference › Paper › peer-review

Abstract

We have researched gene transfection using hydrogen bonding polymer/DNA complex prepared by ultra high pressure technology. In this study, for effective gene transfection, we investigated the preparation of nano-hydroxyl apatite (HAp)/PVA/DNA complex including nano-scaled HAp particles promoting endosomal escape. Gene transfection was carried out using fluorescent labeled plasmid DNA molecules. After 1hour incubation, the effective cellular uptake of HAp/PVA/DNA complexes comparison with PVA/DNA complex and DNA was observed by fluorescent microscope, and then high transfection efficiency was achieved using HAp/PVA/DNA complexes. These results indicate the effective DNA release from endocytosis.

Original language	English
Number of pages	1
Publication status	Published - Oct 19 2006
Externally published	Yes
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: May 24 2006 → May 26 2006

Other

Other	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	5/24/06 → 5/26/06

Keywords

Hydrogen bond
Hydroxyl apatite
Inorganic nano-particles
Nano-composite / gene delivery
Ultra high pressure

ASJC Scopus subject areas

Engineering(all)

Cite this

@conference{7ff2dc727c544c62bef15c1e545b95a7,

title = "Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape",

abstract = "We have researched gene transfection using hydrogen bonding polymer/DNA complex prepared by ultra high pressure technology. In this study, for effective gene transfection, we investigated the preparation of nano-hydroxyl apatite (HAp)/PVA/DNA complex including nano-scaled HAp particles promoting endosomal escape. Gene transfection was carried out using fluorescent labeled plasmid DNA molecules. After 1hour incubation, the effective cellular uptake of HAp/PVA/DNA complexes comparison with PVA/DNA complex and DNA was observed by fluorescent microscope, and then high transfection efficiency was achieved using HAp/PVA/DNA complexes. These results indicate the effective DNA release from endocytosis.",

keywords = "Hydrogen bond, Hydroxyl apatite, Inorganic nano-particles, Nano-composite / gene delivery, Ultra high pressure",

author = "Yoichi Nibe and Tsuyoshi Kimura and Kwangwoo Nam and Shingo Mutsuo and Hidekazu Yoshizawa and Masahiro Okada and Tsutomu Furuzono and Toshiya Fujisato and Akio Kishida",

year = "2006",

month = oct,

day = "19",

language = "English",

note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

}

TY - CONF

T1 - Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape

AU - Nibe, Yoichi

AU - Kimura, Tsuyoshi

AU - Nam, Kwangwoo

AU - Mutsuo, Shingo

AU - Yoshizawa, Hidekazu

AU - Okada, Masahiro

AU - Furuzono, Tsutomu

AU - Fujisato, Toshiya

AU - Kishida, Akio

PY - 2006/10/19

Y1 - 2006/10/19

N2 - We have researched gene transfection using hydrogen bonding polymer/DNA complex prepared by ultra high pressure technology. In this study, for effective gene transfection, we investigated the preparation of nano-hydroxyl apatite (HAp)/PVA/DNA complex including nano-scaled HAp particles promoting endosomal escape. Gene transfection was carried out using fluorescent labeled plasmid DNA molecules. After 1hour incubation, the effective cellular uptake of HAp/PVA/DNA complexes comparison with PVA/DNA complex and DNA was observed by fluorescent microscope, and then high transfection efficiency was achieved using HAp/PVA/DNA complexes. These results indicate the effective DNA release from endocytosis.

AB - We have researched gene transfection using hydrogen bonding polymer/DNA complex prepared by ultra high pressure technology. In this study, for effective gene transfection, we investigated the preparation of nano-hydroxyl apatite (HAp)/PVA/DNA complex including nano-scaled HAp particles promoting endosomal escape. Gene transfection was carried out using fluorescent labeled plasmid DNA molecules. After 1hour incubation, the effective cellular uptake of HAp/PVA/DNA complexes comparison with PVA/DNA complex and DNA was observed by fluorescent microscope, and then high transfection efficiency was achieved using HAp/PVA/DNA complexes. These results indicate the effective DNA release from endocytosis.

KW - Hydrogen bond

KW - Hydroxyl apatite

KW - Inorganic nano-particles

KW - Nano-composite / gene delivery

KW - Ultra high pressure

UR - http://www.scopus.com/inward/record.url?scp=33749842060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749842060&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33749842060

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Gene delivery using nano-HAP/PVA/DNA complexes promoting endosomal escape

Abstract

Other

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this