Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo

Research output: Chapter in Book/Report/Conference proceedingChapter


Dental pulp cells (DPCs) differentiate into odontoblasts. To observe odontoblastic differentiation, the detection of dentinogenesis-specific molecules such as dentin sialophosphoprotein (DSPP) and the measurement of alkaline phosphatase (ALP) activity are reliable approaches. CCN family member 2 (CCN2) has been proposed as a marker for dentinogenesis. Our recent study revealed that the expression levels of Ccn4, Ccn5, and Ccn6 were changed in accordance with odontoblastic differentiation. Therefore, Ccn4, Ccn5, and Ccn6, as well as Ccn2, could serve as a comprehensive set of markers for dentinogenesis. Here, we describe a method of measuring the Ccns expression levels in differentiating rat DPCs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Dental pulp cells
  • Dentinogenesis
  • Odontoblasts
  • Rat teeth
  • Real-time PCR
  • Western blot

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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