TY - JOUR
T1 - Gene Profiles during Root Canal Treatment in Experimental Rat Periapical Lesions
AU - Martinez, Zulema Rosalia Arias
AU - Naruishi, Koji
AU - Yamashiro, Keisuke
AU - Myokai, Fumio
AU - Yamada, Teruo
AU - Matsuura, Kaori
AU - Namba, Naoko
AU - Arai, Hideo
AU - Sasaki, Junzo
AU - Abiko, Yoshimitsu
AU - Takashiba, Shogo
N1 - Funding Information:
We thank Dr. K. Yamabe and Dr. O. Rodis for technical support. This study was supported by Grants-in-Aid for Scientific Research (A) (No. 16209056, No. 18209057, and No. 16209063) from the Japan Society for the Promotion of Science.
PY - 2007/8
Y1 - 2007/8
N2 - The purpose of this study was to profile gene expression in periapical lesions during root canal treatment (RCT). Periapical lesions were induced experimentally by exposing the pulp in Sprague-Dawley rats. After 3 wk, the animals received root canal filling (RCF) and were sacrificed 1 or 4 wk later. From the periapical tissues, total RNA was extracted and processed for cDNA-microarray analysis. The lesions were histologically and radiographically confirmed to expand 4 wk after pulp exposure (inflammation phase) and to stabilize 4 wk after RCF (healing phase). In approximately 30,000 genes on the microarray, 203 genes were up-regulated to more than 5-fold (e.g., IL-1β), and 864 genes were down-regulated to less than 20% of baseline level (e.g., caspase 8) in inflammation phase. Compared with inflammation phase, we found that 133 genes were up-regulated (e.g., IL-1α) and 50 genes were down-regulated (e.g., defensin α5) in healing phase. Corresponding to the gene expression profiles, accumulation of IL-1α and IL-1β was observed in the periapical lesions by immunohistochemistry. These gene profiles might be useful in diagnosing the healing process of periapical lesions.
AB - The purpose of this study was to profile gene expression in periapical lesions during root canal treatment (RCT). Periapical lesions were induced experimentally by exposing the pulp in Sprague-Dawley rats. After 3 wk, the animals received root canal filling (RCF) and were sacrificed 1 or 4 wk later. From the periapical tissues, total RNA was extracted and processed for cDNA-microarray analysis. The lesions were histologically and radiographically confirmed to expand 4 wk after pulp exposure (inflammation phase) and to stabilize 4 wk after RCF (healing phase). In approximately 30,000 genes on the microarray, 203 genes were up-regulated to more than 5-fold (e.g., IL-1β), and 864 genes were down-regulated to less than 20% of baseline level (e.g., caspase 8) in inflammation phase. Compared with inflammation phase, we found that 133 genes were up-regulated (e.g., IL-1α) and 50 genes were down-regulated (e.g., defensin α5) in healing phase. Corresponding to the gene expression profiles, accumulation of IL-1α and IL-1β was observed in the periapical lesions by immunohistochemistry. These gene profiles might be useful in diagnosing the healing process of periapical lesions.
KW - Gene profiles
KW - IL-1α
KW - IL-1β
KW - periapical lesion
KW - root canal treatment
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U2 - 10.1016/j.joen.2007.04.016
DO - 10.1016/j.joen.2007.04.016
M3 - Article
C2 - 17878078
AN - SCOPUS:34447574987
SN - 0099-2399
VL - 33
SP - 936
EP - 943
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 8
ER -