TY - JOUR
T1 - Gene profiling in human periodontal ligament fibroblasts by subtractive hybridization
AU - Yamamoto, T.
AU - Myokai, F.
AU - Nishimura, F.
AU - Ohira, T.
AU - Shiomi, N.
AU - Yamashiro, Keisuke
AU - Arai, H.
AU - Murayama, Y.
AU - Takashiba, S.
PY - 2003/8
Y1 - 2003/8
N2 - Genes expressed by human periodontal ligament fibroblasts (HPFs) are likely to be associated with specific functions of the ligament. The aim of this study is to profile genes expressed highly by HPFs. A library (6 × 10 3 pfu) was constructed, followed by subtraction of HPF cDNAs with human gingival fibroblast (HGF) cDNAs. Reverse-dot hybridization revealed that 33 clones expressed higher levels of specific mRNAs in HPFs than in HGFs. These were mRNAs for known genes, including several associated with maturation and differentiation of cells. None had been reported in PFs. One clone, PDL-29, identified as a COX assembly factor, showed much stronger mRNA expression in HPFs than in HGFs in culture. In rat periodontium, however, PDL-29 mRNA expression was similar in PFs and GFs. These results suggest that HPFs express many previously unreported genes associated with maturation and differentiation, but expression can differ in vitro and in vivo.
AB - Genes expressed by human periodontal ligament fibroblasts (HPFs) are likely to be associated with specific functions of the ligament. The aim of this study is to profile genes expressed highly by HPFs. A library (6 × 10 3 pfu) was constructed, followed by subtraction of HPF cDNAs with human gingival fibroblast (HGF) cDNAs. Reverse-dot hybridization revealed that 33 clones expressed higher levels of specific mRNAs in HPFs than in HGFs. These were mRNAs for known genes, including several associated with maturation and differentiation of cells. None had been reported in PFs. One clone, PDL-29, identified as a COX assembly factor, showed much stronger mRNA expression in HPFs than in HGFs in culture. In rat periodontium, however, PDL-29 mRNA expression was similar in PFs and GFs. These results suggest that HPFs express many previously unreported genes associated with maturation and differentiation, but expression can differ in vitro and in vivo.
KW - Gene profiling
KW - In situ hybridization
KW - Periodontal ligament fibroblasts
KW - Subtractive hybridization
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U2 - 10.1177/154405910308200814
DO - 10.1177/154405910308200814
M3 - Article
C2 - 12885851
AN - SCOPUS:0041384416
SN - 0022-0345
VL - 82
SP - 641
EP - 645
JO - Journal of dental research
JF - Journal of dental research
IS - 8
ER -