TY - JOUR
T1 - Generation of four induced pluripotent stem cell lines (FHUi003-A, FHUi003-B, FHUi004-A and FHUi004-B) from two affected individuals of a familial neurohypophyseal diabetes insipidus family
AU - Yoshida, Satoru
AU - Okura, Hanayuki
AU - Suga, Hidetaka
AU - Soen, Mika
AU - Kawaguchi, Yohei
AU - Kurimoto, Junki
AU - Miyata, Takashi
AU - Takagi, Hiroshi
AU - Arima, Hiroshi
AU - Fujikawa, Tatsuya
AU - Otsuka, Fumio
AU - Matsuyama, Akifumi
N1 - Funding Information:
We thank the patients for participating in this work. We also thank Tomohiko Nishitomi for technical assistance. This work was supported by grants from the Acceleration Program for Intractable Diseases Research utilizing Disease-specific iPS cells of the Research Center Network for Realization of Regenerative Medicine (RCNRRM) funded by Japan Agency for Medical Research and Development ( AMED , Japan, Grant Number JP19bm0804011 ). We would like to thank Editage (www.editage.com) for English language editing.
Publisher Copyright:
© 2020 The Authors
PY - 2020/10
Y1 - 2020/10
N2 - Four disease-specific induced pluripotent stem cell (iPSC) lines were respectively derived from peripheral blood mononuclear cells of two affected individuals in a family affected by familial neurohypophyseal diabetes insipidus carrying the c.314G>C mutation. The expression of pluripotency markers (NANOG, OCT4, and SOX2), maintenance of a normal karyotype, absence of episomal vectors used for iPSC generation, and presence of the original pathogenic mutation were confirmed for each iPSC line. The ability to differentiate into three germ layers was confirmed by a teratoma formation assay. These iPSC lines can help in disease recapitulation in vitro using organoids and elucidation of disease mechanisms.
AB - Four disease-specific induced pluripotent stem cell (iPSC) lines were respectively derived from peripheral blood mononuclear cells of two affected individuals in a family affected by familial neurohypophyseal diabetes insipidus carrying the c.314G>C mutation. The expression of pluripotency markers (NANOG, OCT4, and SOX2), maintenance of a normal karyotype, absence of episomal vectors used for iPSC generation, and presence of the original pathogenic mutation were confirmed for each iPSC line. The ability to differentiate into three germ layers was confirmed by a teratoma formation assay. These iPSC lines can help in disease recapitulation in vitro using organoids and elucidation of disease mechanisms.
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U2 - 10.1016/j.scr.2020.101960
DO - 10.1016/j.scr.2020.101960
M3 - Article
C2 - 32927425
AN - SCOPUS:85090575438
SN - 1873-5061
VL - 48
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 101960
ER -
